Developmental Physiology of Migratory Neurons in the Moth Enteric Nervous System

发育神经生物学的一个中心问题是 因子参与离子通道的分化， 发育中的神经元 这个问题将在迁移中进行研究 在蛾体内形成肠神经系统的神经元， 曼杜卡 该系统非常适合此类研究，因为 这些神经元的发育命运被很好地表征， 这个系统只有300个神经元， 可用于电生理记录和实验 胚胎中的操纵。 此外，这些神经元可以 从胚胎中取出并进行培养研究。 细胞内 并将对确定的 原位和培养迁移期间和之后的神经元 研究电压依赖性的表达和发展， 钾、钙依赖性钾、钠和钙 水流 这些研究将在未来导致分子和 通道基因表达的遗传分析。

A central issue in developmental neurobiology concerns what factors are involved in the differentiation of ion channels in developing neurons. This issue will be studied in migrating neurons that form the enteric nervous system in the moth, Manduca. This system is uniquely suited for such studies since the developmental fates of these neurons are well characterized, there are only 300 neurons in this system and these are easily accessible for electrophysiological recording and experimental manipulation in the embryo. In addition, these neurons can be removed from the embryo and studied in culture. Intracellular and patch clamp recordings will be performed on identified neurons during and after their migration in situ and in culture to study the expression and development of voltage dependent potassium, calcium dependent potassium, sodium and calcium currents. These studies will lead in the future to molecular and genetic analyses of channel gene expression.