Crystal Structure of the Immunity Protein and its Complex with Colicin E3

大肠杆菌素E3是由大肠杆菌的某些菌株分泌的一种毒性蛋白。 大肠杆菌，以消除相关菌株的细菌生活 在同一个生态位。 大肠杆菌素E3灭活 通过切口感染细胞的蛋白质生物合成机制 在特定位点的16 S核糖体RNA。 大肠杆菌素E3因此起作用 作为核糖核酸内切酶。 生产有机体 由于其自身的大肠杆菌素E3的毒性， “免疫蛋白”结合大肠杆菌素E3，从而使大肠杆菌素E3 它不活跃。 Shoham博士提出了一种晶体结构测定方法 大肠杆菌素E3及其抑制剂，免疫蛋白获得 在分子水平上理解核糖核酸酶的作用 大肠杆菌素E_3及其对免疫蛋白的抑制作用。 详细 酶-抑制剂相互作用的知识也将 光对核糖体的结构在大，在 切割现场附近。 免疫蛋白是一种 很少有已知的核糖核酸酶抑制剂。 其三维 结构可以为其他RNA酶的设计提供基础 具有所需特异性的抑制剂。

Colicin E3 is a toxic protein secreted by certain strains of E. coli in order to eliminate related strains of bacterial living in the same ecological niche. Colicin E3 inactivates the protein biosynthetic machinery of the infected cell by nicking 16S ribosomal RNA at a specific site. Colicin E3 thus acts enzymatically as an endoribonuclease. The producing organism is immune to the toxicity of its own colicin E3 by virtue of a "immunity protein" which binds to colicin E3 and thus renders it inactive. Dr. Shoham proposed a crystallographic structure determination of colicin E3 and its inhibitor, the immunity protein gain understanding at the molecular level of the ribonuclease action of colicin E3 and its inhibition of immunity protein. Detailed knowledge of the enzyme-inhibitor interactions will also shed light on the structure of the ribosome at large, in the vicinity of the cutting site. the immunity protein is one the very few known ribonuclease inhibitors. Its three-dimensional structure may provide a basis for the design of other RNAse inhibitors with desired specificity.