Molecular Genetics of Fertilization in Arabidopsis

细胞-细胞识别是一个基本的生物学过程， 是许多重要的发展事件的基础。的示例 这一过程严重依赖于细胞间的相互作用， 高等植物的受精作用本文中描述的研究 建议旨在促进我们对 参与这些细胞间相互作用的机制，如 以及增加我们对这些相互作用的了解 来调节受精过程遗传学方法将是 用于鉴定拟南芥中的突变， 受精的过程和这些突变 其特征在于确定哪些参与细胞-细胞 交互.通过突变识别的基因将是 使用RFLP作图和“染色体”相结合的方法克隆 行走”。克隆的基因将被用来表征 基因的表达模式和产物。以平行 项目，一个小家族的拟南芥基因显示序列 类似于那些参与自我认知的人， 甘蓝施肥的特点是， 确定家庭成员是否在其他方面发挥作用 受精过程中的识别事件。通过识别和 表征作为相互作用的一部分的基因产物 在这个过程中，我们将了解这些分子机制， 交互.

Cell-cell recognition is a fundamental biological process which underlies many critical developmental events. An example of a process which relies heavily on cell-cell interactions is fertilization in higher plants. The research described in this proposal is designed to contribute to our understanding of the mechanisms which are involved in these cell-cell interactions, as well as to increase our knowledge of how these interactions act to regulate the fertilization process. A genetic approach will be used to identify mutations in Arabidopsis thaliana which disrupt the process of fertilization and these mutations will be characterized to determine which are involved in cell-cell interactions. The genes identified by the mutations will be cloned using a combination of RFLP mapping and "chromosome walking". The cloned genes will then be used to characterized the expression patterns and products of the genes. In a parallel project, a small family of Arabidopsis genes showing sequence similarity to those involved in self-recognition during fertilization in Brassica oleracea will be characterized to determine if any members of the family play a role in other recognition events during fertilization. By identifying and characterizing gene products which are part of the interaction process we will learn about the molecular mechanisms of these interactions.