Nitrogen Use Efficiency As A Strategy For Drought AdaptationIn Beans

这项规划拨款提案旨在评估一种非破坏性采样技术的准确性和精密度，该技术将使研究人员能够通过定期从单个植物的相同叶片中切除叶片来获取关于N的分配和再动员的数据。这可以大大降低时间进程15N实验在空间和劳动力方面的成本，同时极大地促进信息的获取，以帮助评估N在植物策略中的作用，从而提高生产力和对压力环境的抗性。建议的方法将促进这一领域的研究，并可与14C同时使用，以便能够研究不同环境、基因型和物种中的C和N分配和再动员。这项技术将在田间和受控环境中使用，并可能被证明是一种对植物育种有用的有价值的筛选工具，如果发现N的分配和再动员是提供对水分胁迫的普遍适应的策略之一或者是在经历了特定类型干旱的环境中。

This planning grant proposal is designed to evaluate the accuracy and precision of a non-destructive sampling technique that would enable researchers to acquire data on N partitioning and remobilization by periodically excising leaf discs from the same leaves of individual plants. This could substantially reduce the cost of time-course 15N experiments in terms of space and labor, while greatly facilitating the acquisition of information to help assess the role of N in plant strategies which result in increased productivity and resistance to stressful environments. The proposed method would promote research in this area and could be used simultaneously with 14C to enable study of C and N partitioning and remobilization in diverse environments, genotypes and species. The technique would be usable in field and controlled environments and could prove to be a valuable screening tool useful with plant breeding if N partitioning and remobilization are found to be one of the strategies which provide adaptation to moisture stress either universally or in environments that experience a specific type of drought.