Instrumentation for Enhancing Undergraduate Genetics, Immunology and Microbiology Laboratories and Student Research

在生物学课程是加强通过收购一个超离心机和动态微孔板阅读器。这些设备正在遗传学、免疫学和微生物学课程中使用，以引入采用现代技术和批判性思维原则的新练习。该设备增加了学生在“研究方法”课程和本科生研究项目中可以进行的项目的多样性。超离心机使学生能够纯化质DNA，用于限制性内切酶双酶切定位和基因克隆，并纯化原核和真核基因组DNA和RNA，用于非放射性标记探针的Southern和Northern blot分析。学生使用动态微孔板阅读器进行ELISA免疫分析，使用MTT试验进行细胞增殖研究，进行单克隆抗体分析，进行Lowry蛋白试验，以及在体内或体外免疫刺激程序后筛选血清和培养上清以确定抗体的存在。这些设备改善了提供给生物学或相关健康专业学生的生物学课程，其中许多是女性。学院将提供等额的奖金。

The curriculum in biology is being enhanced through the acquisition of an ultracentrifuge and kinetic microplate reader. The equipment is being used in the Genetics, Immunology and Microbiology courses to introduce new exercises which employ modern techniques and principles of critical thinking. The equipment increases the variety of projects students may undertake in the Methods in Research course and in undergraduate student research projects. The ultracentrifuge enables students to purify plasmic DNA for restriction endonuclease double digestion mapping and gene cloning, and to purify prokaryotic and eukaryotic genomic DNA and RNA for Southern and Northern blot analyses with non-radioactive labeled probes. The kinetic microplate reader is used by students for performing the ELISA immunoassay, for cell proliferation studies using the MTT assay, for monoclonal antibody analyses, for the Lowry protein assay and for the screning of sera and cultured supernatants for the presence of antibody following immunological in vivo or in vitro stimulation procedures, respectively. The equipment improves the biology program being offered to students majoring in biology or allied health, many of whom are women. The college will contribute an amount equal to the award.