Acquisition of a Beta Scanner

我们申请资金用于beta扫描仪， 定量分析放射性标记（P32、S35和C14） 核酸和蛋白质印迹。 我们将建立一个联合 该设施将主要服务于七个实验室，五个在 生物系和生物化学系各两名。 所要求的仪器将用于实现以下目标 目前资助的项目。 所有的研究工作 实验室有一个非常强大的分子方法学组成部分。 主要的研究工作包括阐明重组 酵母和大肠杆菌中的转录调控机制， 在果蝇中，免疫球蛋白基因控制DNA 非洲爪蟾的复制，果蝇的分子遗传学 神经发育和基因检测方法的发展 隔离 有几个项目特别强调蛋白质- 核酸相互作用 拟议的文书将加强 正在进行的工作的定量精度，并将使 可能的实验太耗时， 仪器. 同样重要的是，拟议的文书将 使我们能够计划以前不切实际的实验 由于在测量中所需的高精度， 数据解释。

We request funds for a beta-scanner with the capability to quantitatively analyze radioactively labelled (P32, S35, and C14) nucleic acid and protein blots. We will establish a joint facility that will primarily serve seven laboratories, five in the Biology department and two in the Biochemistry department. The requested instrumentation will be used to accomplish goals of currently funded projects. The research effort in all laboratories has a very strong molecular methodology component. Major research efforts involve elucidation of recombination mechanisms in yeast and E.coli, transcriptional regulation in Drosophila, and in immunoglobulin genes, control of DNA replication in Xenopus, molecular-genetics of Drosophila neurodevelopment, and development of methodology for gene isolation. Several projects have a special emphasis o protein- nucleic acid interactions. The proposed instrument will enhance the quantitative precision of the ongoing work, and will make possible experiments that are too time consuming with existing instruments. Equally important, the proposed instrument will enable us to plan experiments that were previously unrealistic because of the high degree of precision necessary in the interpretation of data.