Modulation of Oxidative Cross-linking of Plant Cell Wall Structural Proteins: A Novel Site of Regulation in Development and Stress Resistance

激发子处理豆类或大豆细胞悬浮培养物会导致植物细胞壁中原有结构蛋白的快速交联，随后植物细胞表面会发生氧化代谢和过氧化氢的产生。氧化交联也受到与细胞成熟相关的发育控制，例如在下胚轴生长期间，以及在机械应力下的组织中，例如茎-叶柄连接。植物细胞壁中原有结构蛋白的刺激依赖氧化交联是一个新的细胞调控部位，在植物发育过程中灵活地实现细胞壁的最终功能结构，以及在微生物攻击或相关胁迫的初始阶段快速增韧细胞壁具有潜在的重要功能。此外，对特定激发子的快速反应在1-2分钟内开始，并在5-10分钟内完成，为感知和转导激发子信号的最初步骤的功能剖析提供了基础。本提案旨在探讨氧化交联剂的调节、潜在的信号机制以及交联剂对细胞壁特性的影响。

Elicitor treatment of bean or soybean cell suspension cultures causes rapid cross-linking of preexisting structural proteins in the plant cell wall, following a burst of oxidative metabolism and generation of hydrogen peroxide at the plant cell surface. Oxidative cross-linking is also under developmental control associated with cellular maturation, eg., during hypocotyl growth, and in tissues subject to mechanical stress, eg., the stem-petiole junction. Stimulus-dependent oxidative cross-linking of pre- existing structural proteins in the plant cell wall is a novel site of cellular regulation, with potentially important functions in the flexible realization of the final functional architecture of cell walls during plant development, and rapid toughening of walls in the initial stages of microbial attack or related stresses. Moreover, the rapid response to defined elicitors, which is initiated within 1-2 minutes and complete within 5-10 minutes, provides the basis for functional dissection of the initial steps in the perception and transduction of elicitor signals. The present proposal seeks to explore the regulation of oxidative cross-linking, underlying signal mechanisms and the impact of cross-linking on cell wall properties.//