Neural Regulation of Muscle Cell Interaction During Development

转换在慢和快肌肉运动单位形成中的作用将在鸡胚胎中进行研究。在次级肌管从初级肌管簇分离的过程中，细胞表面分子NCAM和N-钙粘附素的变化将被研究。这些细胞表面分子的功能作用将在卵子、雏鸡和使用封闭抗体或酶降解的细胞培养中进行研究。最后，在一系列的移植研究中，将探讨肌管簇形成、突触形成和功能神经支配的作用。在这一系列移植研究中，将小块的鹌鹑神经管移植到鸡体内。这些研究在方法上是独一无二的，因为它们将分子变化和细胞-细胞关系与神经功能的开始相关。

The role of innvervation in slow and fast muscle-motor unit formation will be studied in the chick embryo. Changes in NCAM and N-cadherin, cell surfaces molecules, in the process during which secondary myotubes partition off from primary myotube clusters will be investigated. The functional role of these cell surface molecules will be studied in ovo in the chick and in cell culture using blocking antibodies or enzymatic degradation. Finally, the roles of myotube cluster formation, synapse formation and functional innervation will be investigated in a series of transplantation studies in which small pieces of quail neural tube are transplanted into chick. These studies are unique in their approach in that they correlate molecular changes and cell-cell relationships with onset of neural function.