Collaborative Project: Application of Spin Trapping to the Study fo Netrophil Phagsomal Events

本研究的总体目标是提高自旋捕获在吞噬细胞中的应用，更好地了解吞噬细胞的自由基生物学。利用自旋陷阱4- POBN，研究人员已经证明，在myloperoxidase介导的反应中，形成的1-3%的氧自由基可以作为羟基自由基被捕获。这些结果可能会解决文献中相互矛盾的问题。下一个目标是完成4-POBN的工作。第二个目标是澄清自旋捕获剂的定位。这将通过使用荧光自旋捕获探针与共聚焦显微镜来确定。最后，在接下来的一年中，将完成对乳铁蛋白在羟基自由基形成中的作用的研究。这项研究将继续发展自旋捕获技术在生物系统研究中的应用，并将有助于理解吞噬细胞内的自由基事件。

The overall goals of this research are to improve the application of spin trapping to phagocyte cells and to better understand the free radical biology of phagocytic cells. Using the spin trap 4- POBN, the investigators have demonstrated that 1-3% of oxygen radical formed can be trapped as hydroxyl radical in a myloperoxidase-mediated reaction. These results will likely resolve a conflicting body of literature. The next aim will be to complete work with 4-POBN. A second goal is to clarify the localization of spin trapping agents. This will be determined by using fluorescent spin trapping probes with confocal microscopy. Finally, during the course of the coming year, studies of the role of lactoferrin in hydroxyl radical formation will be completed. This research will continue development of techniques for the application of spin trapping to the study of biological systems and will contribute to understanding of free radical events within phagocytic cells.