Improvement in Instrumentation and Equipment for Teaching Cellular and Molecular Biology at Saddleback College

项目概述：现代生物课程应熟悉 学生们从细胞的概念和方法， 和分子生物学。目前在马鞍峰学院， 学生有机会探索原核生物， 使用标准化合物的真核细胞结构， 解剖显微镜，以及观察细胞结构 使用扫描和透射电子显微镜。我们 我喜欢增加细胞结构和功能的教学 通过细胞的分离和分析， 离心、蛋白质凝胶电泳、核酸凝胶 电泳和紫外光谱。本设备 可以让我们分离出细胞器和大分子 在我们的细胞生物学，微生物学和入门生物学中 实验室课程，并进行简单的练习 重组DNA技术。购买一台冷藏的 高速离心机、台式微型离心机、蛋白质和 核酸电泳凝胶盒，电泳功率，电泳时间 紫外分光光度计附件，我们 我觉得我们将加强和更新我们的实验室， 细胞生物学和微生物学课程。由于许多 细胞生物学和微生物学课程的学生转到 四年制大学完成他们的 学士学位，他们接触的技术 现代细胞和分子生物学将改善他们的未来 对于学业成功，特别是对于那些 转入研究型大学。而且我们 计划将这些技术的某些方面引入到 普通生物学入门课程和非专业生物学 课程，以提高科学素养的 这些学生在现代分子生物学领域。

Project Summary: The modern biology curriculum should acquaint students with concepts and methodologies derived from cellular and molecular biology. Currently at Saddleback College, students have the opportunity to explore prokaryotic and eukaryotic cell structure using standard compound and dissecting microscopes, as well as viewing cell structures using scanning and transmission electron microscopes. We would like to augment the teaching of cell structure and function through the fractionation and analysis of cells using centrifugation, protein gel electrophoresis, nucleic acid gel electrophoresis, and ultraviolet spectroscopy. This equipment will allow us to isolate cellular organelles and macromolecules in our cell biology, microbiology and introductory biology laboratory courses, and to perform simple exercises in recombinant DNA technology. With the purchase of a refrigerated high speed centrifuge, tabletop mini-centrifuges, protein and nucleic acid electrophoresis gel boxes, electrophoresis power supplies, and ultraviolet spectrophotometer attachments, we feel that we will strengthen and update our laboratory curriculum in cell biology and microbiology. Since many of the students in cell biology and microbiology courses transfer to four year colleges and universities to complete their baccalaureate degrees, their exposure to the techniques of modern cellular and molecular biology will enhance their future for academic success, particularly for those students who transfer to research-oriented universities. Furthermore, we plan to introduce some aspects of these techniques to introductory general biology courses and non-majors biology courses in an effort to increase the scientific literacy of these students in the areas of modern molecular biology.