Collaborative: Characterization and Role of Spitzenkorper in Hyphal Tip Growth and Morphogenesis

这个合作项目整合了细胞学的关键方面， 生理学和生物化学来解决一个主要问题， 发育生物学：解释最基本的生长 真菌中菌丝顶端生长的过程。 菌丝拉长一 复杂的机制，涉及运动的壁建设囊泡， 顶点 在高等真菌中，囊泡聚集在菌丝顶端， 形成了一个独特的复合体，叫做斯皮森科尔珀 Spitzenkorper 被怀疑在顶端生长中起核心作用，但其 控制其作用的确切功能和机制仍然存在 未知 通过相关视频增强光学显微镜的生长 细胞、电子显微镜、细胞化学和免疫细胞化学， 研究将描述Spitzenkorper组织特征， 行为 顶端囊泡的表征将允许 确定它们在细胞生长中的生物化学作用。 发育突变体将被用来关联Spitzencorper 行为与菌丝生长，并提供线索， 控制极性和顶端生长。//

This collaborative project integrates key aspects of cytology, physiology, and biochemistry to resolve a major problem in developmental biology: explaining the most fundamental growth process in fungi -- apical growth of hyphae. Hyphae elongate by a complex mechanism involving movement of wall-building vesicles to the apex. In higher fungi, vesicles assemble in the hyphal tip to form a unique complex called the Spitzenkorper. The Spitzenkorper is suspected of playing a central role in apical growth, but its exact function and mechanisms controlling its action remain unknown. By correlation video-enhanced light microscopy of growing cells, electron microscopy, cytochemistry, and immunocytochemistry, the research will characterize Spitzenkorper organization and behavior. Characterization of apical vesicles will allow determination of their biochemical roles in cell growth. Developmental mutants will be used to correlate Spitzenkorper behavior with hyphal growth and provide clues concerning the control of polarity and apical growth. //