Acquisition of a Scanning Electron Microscope.

本提案要求拨款购买一台扫描电子显微镜和一台x射线微量分析系统。北达科他州立大学的电子显微镜中心是一个公共仪器设施，提供校园内唯一的电子显微镜访问。每年通常有70到90名研究科学家和学生使用该设施。除了维护设备外，该实验室还提供一整套服务。在过去的几年里，食品技术专家和谷物化学家发现了对超微结构数据的需求。许多样品，如面团和麸质，特别难以制备用于扫描电子显微镜。有充分的文献证明，用于生物标本的制备程序会导致重要的人工制品形成。冷冻制备和检查的冷冻，充分水合面团和麸质产生最好的数据。样品充电是目前扫描显微镜的一个严重问题，因为在小于8kV的加速电压下通常不可能获得二次图像。为了满足这些科学家目前的研究要求，并模拟持续的食品超微结构研究，购买本提案中列出的设备是必不可少的。

This proposal requests funds for the purchase of a scanning electron microscope and an x-ray microanalysis system. The Electron Microscopy Center at North Dakota State University is a common instrument facility that provides the only access to electron microscopy on campus. There are usually between 70 and 90 research scientists and students that use the facility each year. In addition to maintaining the equipment, the laboratory offers a complete line of service. In the last few years, food technologists and cereal chemists have discovered the need for ultrastructural data. Many samples such as doughs and glutens are particularly difficult to prepare for scanning electron microscopy. It is well documented that the preparation procedures employed for biological specimens result in significant artifact formation. Cryo-preparation and examination of frozen, fully hydrated doughs and glutens yield the best data. Specimen charging is a serious problem with the present scanning microscope as it is not generally possible to obtain secondary images at accelerating voltages of less than 8kV. To meet the present research requirements of these scientists and to simulate continued ultrastructural studies of foods, the acquisition of the equipment listed in this proposal is essential.