Structural Organization of Proteins on the Oxidizing Side of Photosystem II

该建议的目的是：（1）确定受影响者， PSII多肽上的化学敏感氨基酸残基 在选择性地去除外源蛋白的各种处理之后 和异质Mn簇，（2）鉴定能够 （3）描述一个可能的X-连锁的结构， 外源结构域异源三聚体复合物，和（4）使用振动 光谱和圆二色性来预测整体 每个外源蛋白的二级结构。 %%% 光合作用过程中的水氧化（氧气释放）是一种 这个星球上所有生物的关键反应。 的 拟议的研究将描述 菠菜中参与氧气释放的蛋白质。 贡献 对蛋白质-蛋白质和蛋白质-金属离子的理解 这些研究可能会导致 发现合成配合物的形成方法 能够进行大规模的多电子催化。

The aims of this proposal are to: (1) identify the exposed and chemically susceptible amino acid residues on the PSII polypeptides after various treatments to selectively remove extrinsic proteins and heterogeneous Mn clusters, (2) identify protein domains capable of being x-linked, (3) characterize the structure of a possible extrinsic domain hetero-trimer complex, and (4) use vibrational spectroscopy and circular dichroism to predict the overall secondary structure of each extrinsic protein. %%% Water oxidation (oxygen evolution) during photosynthesis is one of the key reactions for all the living creatures on this planet. The studies proposed will characterize the interactions among the proteins involved in oxygen evolution in spinach. In contributing to an understanding of protein-protein and protein-metal ion interactions in living systems these studies may lead to the discovery of approaches to the formation of synthetic complexes capable of multielectron catalysis on large scales.