Regulation of Inositol Metabolism in Arabidopsis

9307092约翰逊，肌醇的生物合成在时间和空间上受拟南芥的控制。由于拟南芥是经典和分子遗传学实验的极佳生物，可以用分子遗传学来研究肌醇代谢的遗传控制方面的问题，以研究关键的生物合成酶，肌醇1磷酸合成酶(MI 1 Psynthase)EC5.5.1.4，它将6磷酸葡萄糖转化为1磷酸肌醇，以及关键的分解代谢酶，肌醇加氧酶EC1.13.99.1，催化肌醇氧化途径的第一步。对这些酶及其编码基因的研究将有助于对植物代谢过程的遗传调控的基本了解。这项研究的长期目标是从机制上确定高等植物肌醇代谢的遗传控制。具体地说，本提案中概述的工作将1)研究(利用克隆的拟南芥MI 1 P合成酶基因)参与MI 1 P合成酶调控表达的机制(S)，2)对MI 1 P合成酶进行亚细胞定位，3)从拟南芥中分离肌醇加氧酶及其结构基因。阐明肌醇代谢的遗传调控机制对于了解植物的生长发育具有重要意义。肌醇是真核细胞中普遍存在的一种成分，在植物的生化和生理过程中起着至关重要的作用。***

9307092 Johnson The biosynthesis of inositol is temporally and spatially controlled in Arabidopsis thaliana . Because Arabidopsis is an excellent organism for experiments in classical and molecular genetics, aspects of the genetic control of inositol metabolism can be defined using molecular genetics to study the pivotal biosynthetic enzyme, myoinositol 1 phosphate synthase (MI 1 Psynthase) EC5.5.1.4, which converts glucose 6 phosphate to inositol 1 phosphate and the pivotal catabolic enzyme, myo inositol oxygenase EC1.13.99.1, which catalyzes the first step in the inositol oxidation pathway. Studies of these enzymes and the genes that encode them will contribute to the basic understanding of the genetic regulation of metabolic processes in plants. The long term objective of the research is to define, mechanistically, the genetic control of inositol metabolism in higher plants. Specifically, the work outlined in this proposal will 1)study (using the cloned Arabidopsis MI 1 Psynthase gene) mechanism(s) involved in the regulated expression of MI 1 P synthase, 2) locate MI 1 Psynthase subcelluarly and 3) isolate the inositol oxygenase and its structural gene from Arabidopsis . %%% Elucidation of mechanisms involved in the genetic regulation of inositol metabolism is important for understanding plant growth and development. Inositol, a ubiquitous component of eukaryotic cells, plays a vital role in the biochemistry and physiology of plants. ***