Minority Postdoctoral Research Fellowship

9308049 Rasche申请人将研究醋酸酯转化为甲烷的甲基转移途径。催化特定甲基转移步骤的酶将被纯化和表征。这种酶将产生抗体，免疫金标记将用于在细胞中定位这种酶。他们计划克隆该酶的基因并对其进行测序，并通过搜索合适的数据库来分析推导出的氨基酸序列。这项工作将在弗吉尼亚理工学院和州立大学的James D. Ferry博士的实验室进行。＊＊＊

9308049 Rasche This applicant will study the pathway of methyl transfer in the conversion of acetate to methane. The enzyme catalyzing one of the specific methyl group transfer steps will be purified and characterized. Antibodies will be generated to the enzyme and immunogold labeling will be used to localize the enzyme in the cell. There are plans to clone and sequence the gene(s) for the enzyme, and to analyze the deduced amino acid sequence by searching appropriate databases for similar structures. This work will be conducted in the laboratory of Dr. James D. Ferry at the Virginia Polytechnic Institute and State University. ***