Microscopically Controlled Biomineralization at Genetically Engineered Protein Templates

该项目旨在产生 一个实验性的蛋白质系统 旨在同时开发一种 基础材料加工 能力，并开发一个 基本理解 界面蛋白-无机 晶体分子识别 的 这项工作的主要贡献是 从实验能力到 精确控制 三维空间 羧酸残基分布 在蛋白质表面。 两种蛋白质， 链霉亲和素和蛋白G， 是经过精心挑选的 study. 他们都有一个 β折叠占优势， δ-螺旋二级结构， 因此，提供了一个明确的 脚手架上操作 谷氨酸和 天冬氨酸侧链。 的 分辨率和三维 遗传提供的空间控制 工程能力不能 目前由其他匹配 技术. 这项工作因此 代表了一种独特而强大的 除了实验 分子表征 生物矿化中的识别

This project is aimed at generating an experimental protein system designed to simultaneously develop a fundamental materials processing capability, and to develop a fundamental understanding of interfacial protein-inorganic crystal molecular recognition. The key contribution of this work arises from the experimental capability to precisely control the three-dimensional spatial distribution of carboxylate residues on a protein surface. Two proteins, streptavidin and protein G, have been carefully selected for this study. They both have a predominance of beta-sheet and delta-helix secondary structure, and, thus, provide a defined scaffolding on which to manipulate the placement of glutamic and aspartic acid side chains. The resolution and three-dimensional spatial control provided by genetic engineering capabilities cannot be currently matched by other techniques. This work thus represents a unique and powerful addition to the experimental characterization of molecular recognition in biomineralization.