SGER: Mitosis Promoting Factor, A Possible Measure of Instantaneous Growth Rate in Teleost Larvae

渔业生态学的一项主要努力是开发生化工具，以量化招募鱼苗幼鱼的瞬时增长率和营养动态。最近的研究导致了一系列创新技术的出现。由于这些方法的解释问题，目前招募研究的趋势是结合使用生化、组织学和形态计量学技术。因此，所需要的是一个真正特定的生化指标，以反映细胞在任何时间点的实际生长速度。细胞周期生化调控的最新进展表明，有丝分裂促进因子(MPF)是细胞周期的“主振荡器”。对MPF活性的测量表明，MPF作为一种高度特异的细胞增殖和生长指标具有巨大的潜力。为了测试这一想法，DiMichele将开发分析技术，验证异步细胞种群中致力于有丝分裂的细胞比例，并将理论模型与红鼓中已知的整个动物增长率相匹配。如果该模型得到验证，MPF的生化测量将具有巨大的潜力，作为一种专门测量异质种群中瞬时生长和细胞增殖的指标。

A major effort in fisheries ecology has been to develop biochemical tools for quantifying instantaneous growth rate and the nutritional dynamics of recruiting fish larvae juveniles. Recent studies have lead to a succession of innovative techniques. Due to problems of interpretation with these methods, current trend in recruitment studies is to use combination of biochemical, histological, and morphometric techniques. Thus, what is needed is a truly specific biochemical indicator of the actual rate of cell growth at any point in time. Recent advances in the biochemical regulation of the cell cycle indicate that a protein kinase, Mitosis Promoting Factor (MPF), acts as the "master oscillator" of the cell cycle. Measurement of MPF activity suggest that MPF has tremendous potential as a highly specific measure of cellular proliferation and growth. To test this idea, DiMichele will develop analytical techniques, verify the proportion of cells committed to mitosis in asynchronous cell populations, and fit the theoretical model to known whole animal growth rates in the red drum, Sciaenops ocellatus. If the model is verified, the biochemical measurement of MPF will have a tremendous potential as a specific measure of instantaneous growth and cellular proliferation in heterogeneous populations.