RUI: Regulation of Transcription Factor Expression in Saccharomyces cerevisiae

虽然已知一般转录因子的浓度对基因在体内的表达很重要，但对酵母中编码TFIIB蛋白的SUA7基因的表达却知之甚少。利用基因融合和引物延伸试验来评估启动子活性，我们将研究SUA7在各种条件下的调控。这项研究有望为真核生物启动子结构和一般转录因子对启动子活性的影响提供有趣的信息。细胞调控通常包括控制DNA模板合成RNA。这种类型的调控对维持正常细胞状态至关重要，了解基因调控对医学和生物技术的进步都很重要。尽管我们对DNA中控制基因表达的信号了解很多，但对与DNA相互作用的分子（转录因子）表达的调控却知之甚少。使用酵母作为实验系统，我建议研究基因表达所需的特定转录因子的调节。这项研究将有助于我们对高等生物基因调控机制的理解。＊＊＊

9317062 Hoopes Although it is known that the concentration of the general transcription factors are important to gene expression in vivo, very little is known about the expression of the yeast SUA7 gene, which codes for the TFIIB protein of yeast. Using gene fusions and primer extension assays to assess promoter activity, we will examine the regulation of SUA7 under a variety of conditions. This study promises to provide interesting information both on eukaryotic promoter structure and on the effect of the general transcription factors on promoter activity. %%% Cellular regulation often involves the control of RNA synthesis from the DNA template. This type of regulation is essential to maintaining the normal cell state, and understanding gene regulation is important to advances in both medical science and biotechnology. Although we know much about the signals in the DNA that control gene expression, we know less about the regulation of the expression of the molecules (transcription factors) that interact with the DNA. Using yeast as an experimental system, I propose to examine the regulation of a particular transcription factor required for gene expression. This research will contribute to our understanding of mechanisms of gene regulation in higher organisms. ***