RUI: Acquisition of Inverted and Upright Light Microscopes and an Image Processing and Analysis System to Support Research in Cell Biology

9317873 Koch光显微研究是细胞生物学研究的基石。任何细胞生物学研究实验室都不应该没有完整的现代光学显微镜技术来分析细胞的结构和功能。该提案寻求配套资金购买一台倒置显微镜和一台直立显微镜，每台显微镜都具有相衬、会聚荧光和微分干涉对比光学，具有静止或视频显微摄影功能，所有这些都与最先进的图像采集和分析系统以及专用图像处理计算机相结合。这台仪器将构成一个新的光学显微镜实验室的核心。它的收购继续我们的努力，以提高研究细胞生物学的教师，研究生和本科生的研究机会。所要求的型号的显微镜目前没有现成的。研究人员必须在一个研究级的直立显微镜上占用时间，或者在五个课程中与学生竞争四个学生级的直立显微镜——根本没有研究级的倒置显微镜或图像采集/处理仪器。所要求的仪器将促进被囊精子和卵子活化的细胞生物学研究、海马神经元mRNA的轴突运输、植物对紫外线损伤的保护以及其他五个小项目。

9317873 Koch Light microscopic studies comprise the cornerstone of cell biological investigations. No cell biology research laboratory should be without access to the full complement of modern light microscopy techniques for analyzing cell structure and function. This proposal seeks matching funds to purchase one inverted microscope and one upright microscopes each having phase contrast, epifluorescence and differential interference contrast optics with still or video photomicrography capabilities all to be coupled to a state-of-the-art image acquisition and analysis system and a dedicated image processing computer. This instrumentation will form the nucleus of a new Light Microscopy Laboratory. Its acquisition continues our effort to enhance research opportunities for faculty, graduate and undergraduate students studying cell biology. Microscopes of the types requested are not readily available at present. Researchers must borrow time on a single research-grade upright microscope or compete with students in five courses for four student grade upright microscopes--no research-grade inverted microscope or image acquisition/processing instrumentation is available at all. The requested instrumentation will promote studies of cell biology of tunicate sperm and egg activation, axonal transport of mRNA in sea hare neurons, and protection to UV damage in plants as well as five other minor projects.