Role of the Luminidependens Gene in Floral Induction

植物生命周期的主要发育变化是开花的开始。遗传和生理分析表明，许多植物物种已经进化出了调节开花的能力，以响应环境因素，如温度或白天长度的变化。然而，植物感知和响应环境信号启动开花的生化机制尚不清楚。为了在分子水平上研究这一过程奠定基础，Amasino博士从拟南芥（Arabidopsis thaliana）中分离出了一个参与成花诱导的基因，名为luminidependens （LD）。LD基因突变导致拟南芥晚花；因此，LD的野生型功能可能是促进开花。生理分析表明，LD与光周期有关。Amasino博士建议在mRNA和蛋白质水平上表征LD的组织特异性、发育时间和环境调节，并确定来自其他物种的LD基因序列，并对蛋白质进行定点诱变。为了确定LD在其他物种中的作用，他将通过反义RNA和/或共抑制技术阻断其他物种的表达。Amasino博士将探索不同的光质量和数量对突变型和野生型植物表型的影响，以解决受LD突变影响的光感受器系统。他将确定LD突变与其他开花突变的相互作用，以确定LD在开花途径中的位置。最后,Amasino博士将启动鉴定LD突变抑制因子和分离LD调控基因的长期目标。*** SOL EXE @ j SOUNDRECEXE @ j = i SSMARQUESCR @ j W B SSMYST SCR @ j ' L SSSTARS SCR @ j j ' L TADA WAV @ j j j L TARTAN BMP @ j v TERMINALEXE @ j B TERMINALHLP @ j THATCH BMP @ j v 9318481 Amasino主要发育植物生命周期的变化是开花的开始。基因和生理？​:哦?!!F ?吗?(Times New Roman Symbol & Arial " h Wd + Dana Brigham Dana Brigham

9318481 Amasino The major developmental change in the plant life cycle is the initiation of flowering. Genetic and physiological analyses have revealed that many plant species have evolved the ability to regulate flowering in response to environmental cues such as changes in temperature or daylength. However, the biochemical mechanisms by which plants sense and respond to environmental cues to initiate flowering are unknown. To lay a foundation for studying this process at the molecular level, Dr. Amasino has isolated a gene from Arabidopsis thaliana, luminidependens (LD), that is involved in floral induction. Mutations in the LD gene render Arabidopsis late flowering; thus, the wild-type function of LD is likely to promote flowering. Physiological analyses indicate that LD is specifically involved in photoperiodism. Dr. Amasino proposes to characterize the tissue specificity, developmental timing, and environmental regulation of LD at the mRNA and protein levels, and determine the sequence of LD genes from other species, and perform site-directed mutagenesis on the protein. To determine the role of LD in other species, he will block expression in other species through antisense RNA and/or co-suppression techniques. Dr. Amasino will explore the effects of different light qualities and quantities on the phenotype of mutant and wild-type plants to address the photoreceptor systems influenced by the LD mutation. He will determine the interactions of the LD mutation with other flowering mutations to define the position of LD in flowering pathways. Lastly, Dr. Amasino will initiate the long-term goals of ident ifying suppressors of the LD mutation and isolating genes regulated by LD. *** SOL EXE @ j SOUNDRECEXE @ j = i SSMARQUESCR @ j W B SSMYST SCR @ j ` L SSSTARS SCR @ j j D TADA WAV @ j s l TARTAN BMP @ j v TERMINALEXE @ j B TERMINALHLP @ j THATCH BMP @ j V 9318481 Amasino The major developmental change in the plant life cycle is the initiation of flowering. Genetic and physiolo ? : ? ! ! F ? ? ( Times New Roman Symbol & Arial " h Wd Wd + Dana Brigham Dana Brigham