Transformation of Chickpea with Bt Genes, U.S.-Pakistan Cooperative Research

项目描述：本项目是由两个知名实验室合作进行的研究项目：西雅图华盛顿大学Milton Gordon博士实验室和巴基斯坦拉合尔旁遮普省大学高级分子生物学中心S. Riazuddin博士实验室。该工作旨在开发植物转化程序，该程序将应用于鹰嘴豆，以表达具有对美洲棉铃虫（Heliothis armigera）杀虫特性的苏云金芽孢杆菌杀虫蛋白。这项研究将涉及三个主要目标。首先，在特定启动子的控制下，将从巴基斯坦环境中分离出的Bt杀虫基因克隆到植物表达载体上。其次，将开发利用粒子轰击技术转化鹰嘴豆报告基因（GUS）的方法。最后，将这些方法应用于将鹰嘴豆转化为Bt杀虫基因，并检测转基因植物的转基因表达水平和抗虫性。范围：该项目将为Riazuddin博士实验室的一名年轻科学家提供资金，让他在华盛顿大学度过几个月，与Gordon博士一起使用拉合尔没有的设备进行实验。这项合作研究预计将对两个实验室非常有益，特别是对生产抗虫经济作物的努力有用。＊＊＊

9320083 Gordon Description: This project is for a collaborative research by two well known laboratories: Dr. Milton Gordon's Laboratory, University of Washington, Seattle, and Dr. S. Riazuddin's Laboratory at the Centre for Advanced Molecular Biology, University of Punjab, Lahore, Pakistan. The work seeks to develop plant transformation procedures which will be applied to chickpea for the expression of Bacillus thuringiensis pesticidal proteins possessing entomocidal properties against American bollworm (Heliothis armigera). The study will address three main objectives. Firstly, pesticidal genes identified in Bt isolates from Pakistani environments will be cloned in plant expression vectors under the control of specific promoters. Secondly, procedures will be developed for the transformation of chickpea by a reporter gene (GUS) using particle bombardment. Finally, these procedures will be applied to transform chickpea with Bt pesticidal genes and the transgenic plant will be examined for level expression of the transgene and insect resistance. Scope: The project will provide fund for a young scientist from Dr. Riazuddin Laboratory to spend several months at the University of washington to participate with Dr. Gordon in the experiments using facilities not available in Lahore. This collaborative research is expected to be quite beneficial to the two laboratories, and particularly useful in the efforts towards producing isect-resistant economic crops. ***