Interdisciplinary Applications of Flourescence Spectroscopy

这个项目将加强新英格兰大学学生的本科实验室体验，方法是将荧光光谱学纳入生命科学系为化学、生物学、海洋生物学和环境科学专业学生提供的几门课程和研究经验。由于其对环境和临床重要性问题的敏感性和适用性，荧光的分析应用正在急剧增长，并将在定量分析、仪器方法、生物化学/细胞生物学和海洋生物学/海洋学实验室中得到应用。要进行的分析的例子包括测定药物成分、天然水中的污染物、植物材料中的叶绿素以及用于监测浮游植物和水团迁移的荧光标记。这项技术在生物化学和细胞生物学中的应用越来越多，除了其他应用外，还将在探索细胞内离子传输机制方面进行说明。更多的基础应用将在普通化学中介绍，并在仪器方法和物理化学中详细探索。荧光免疫分析将成为免疫学实验室的一个组成部分。荧光也将被广泛用于本科生的研究。建议的仪器的多学科使用将促进化学和生物学教师之间的合作互动，并给学生留下越来越多的现代科学的跨学科性质的印象。南缅因州大学的学生还将在仪器方法和物理化学课程中使用这些仪器。

This project will enhance undergraduate laboratory experiences for students at the University of New England by incorporating fluorescence spectroscopy into several courses and research experiences offered for Chemistry, Biology, Marine Biology and Environmental Science students within the Division of Life Sciences. The analytical applications of fluorescence are growing dramatically because of its sensitivity and applicability to problems of environmental and clinical importance and will be exploited in Quantitative Analysis, Instrumental Methods, Biochemistry/Cell Biology and Marine Biology/Oceanography laboratories. Examples of analyses to be performed include the determination of pharmaceutical components, pollutants in natural waters, chlorophyll in plant materials and fluorescent markers for monitoring phytoplankton and water mass migration. The technique's increasing use in Biochemistry and Cell Biology will be illustrated in, among other applications, probing intracellular ion transport mechanisms. More fundamental applications will be introduced in General Chemistry and explored in detail in Instrumental Methods and Physical Chemistry. Fluorescence immunoassay will become a component of the Immunology lab. Fluorescence will also be used extensively for undergraduate research. The multidisciplinary use of the proposed instrument will foster increased cooperative interactions between Chemistry and Biology faculty and impress upon students the increasingly interdisciplinary nature of modern science. The instruments will also be used by University of Southern Maine students in Instrumental Methods and Physical Chemistry courses.