Scanning Galvanometer Excitation System

93-61464火焰 高荧光阳离子特异性探针的出现与视频成像技术相结合，导致了对单个活细胞中离子分布的光度测量的兴趣。 特别感兴趣的是利用荧光染料Fura-2和双波长比率成像定量细胞溶质游离钙浓度（Ca 2 + 1）。 当前遇到的技术困难之一，但Ca 2 + i比率成像的重要目标，是执行真实的时间成像（即每秒30帧的视频帧速率）的能力。 当使用Fura-2时，跟随Ca 2 + i的快速变化的能力可能受到激发波长被截断的速率的限制。 如果要在真实的时间内进行比值，则要求在视频信号（1200）的垂直消隐周期内切换激发波长。 本计画的目的是发展一套高速紫外波长切换（激发）系统，以进行神经系统的真实的时间光学成像。 第一阶段的提案将专门讨论利用双扫描检流计系统在多个干涉滤光片之间切换的可行性。 ***

93-61464 Flaming The advent of highly fluorescent cation specific probes coupled with video imaging technologies has led to a sure of interest in photometric measurement of ion distributions in single living cells. Of particular interest has been the quantitation of cytosolic fee calcium concentrations ( Ca2+ i) utilizing the fluorescent dye Fura-2 and dual wavelength ratio imaging. One of the current technical difficulties encountered, but an important goal for Ca2+ i ratio imaging, is the ability to perform real- time imaging (i.e. video frame rates of 30 frames per second). When using Fura-2, the ability to follow fast changes in Ca2+ i can be limited by the rate at which the excitation wavelengths are chopped. If ratioing is to be done in real time, the requirement if that the excitation wavelength be switched within the vertical blanking period of a video signal (1200). The intent of this project is to develop a high speed UV- wavelength switching (excitation) system for real time optical imaging of the nervous system. This Phase I proposal will specifically address the feasibility of utilizing a dual scanning galvanometer system to switch between multiple interference filters. ***