Modification, Functionalization, and Characterization of Carbon-Fiber Electrodes by Scanning Electrochemical Microscopy

该项目得到分析和表面化学计划的支持，重点是应用扫描电化学显微镜(SECM)和光学成像来表征碳纤维表面的异质性，以及通过SECM控制对这些表面进行修饰以产生已功能化的区域。然后，这些微细加工的表面将被用于制造基于酶的不同寻常的氧化还原传感器，这将得到广泛的评估。密西西比州立大学的David Wipf教授和他的学生与加州大学河滨分校的Werner Kuhr教授和他的学生合作，将在这项为期34个月的持续拨款期间追求这些目标。电极表面的化学修饰可以使这些装置作为目标分析物的分子选择性传感器得到独特的应用。碳电极，特别是碳纤维微电极，提供了一个丰富的合成平台，可以在其上构建各种分子特异性的电化学传感器。这项研究的目的是了解用于这些应用的碳纤维表面的异质性，并开发氧化还原酶空间分辨附着到这些表面的方案。这项研究应该导致开发具有广泛生物技术重要性的新的分析探针。

This project, supported in the Analytical and Surface Chemistry Program, focusses on the application of scanning electrochemical microscopy (SECM) and optical imaging to characterizing the heterogeneity of carbon fiber surfaces and the SECM-controlled modification of these surfaces to produce regions which have been functionalized. These microfabricated surfaces will then be used to make unusual redox enzyme-based sensors which will be broadly evaluated. Professor David Wipf and his students at Mississippi State University, working in collaboration with Professor Werner Kuhr and his students at the University of California at Riverside, will pursue these goals during the tenure of this thirty-four month continuing grant. Chemical modification of the surface of electrodes can enable unique applications of these devices as molecularly selective sensors of target analytes. Carbon electrodes, particularly carbon fiber microelectrodes, offer a rich synthetic platform on which to build a wide variety of molecularly specific electrochemical sensors. This research is aimed at understanding the heterogeneity of surfaces of carbon fibers to be used in these applications and the development of protocols for the spatially resolved attachment of redox enzymes to these surfaces. This research should lead to the development of new analytical probes of broad biotechnological importance.