Multi-User Microscope for Analysis of Double-Label Epifluorescence

建议编号：IBN-9418641首席研究员：谢丽尔·西斯克该奖项旨在为密歇根州立大学的研究神经内分泌学家提供高分辨率荧光显微镜。高结果荧光显微镜将被用来将生物分子定位到神经系统特定区域内单个细胞的特定亚室。这些分子被标记了荧光标记，在紫外光下显示特殊的颜色。这样，不同的分子可以用不同的颜色来标记，例如，确定特定的一对分子是一起出现在特定细胞内还是单独出现在特定细胞内。这些科学家将使用显微镜同时检测活性依赖标记和类固醇受体蛋白，比较肥大细胞和神经胶质细胞中的酶表达模式，以及神经肽和神经递质在大脑不同区域的共存。此外，这项技术可以用来确定当动物参与不同的行为或受到不同的光周期(如季节发生的光周期)时，这些分子的分布是如何变化的。这种显微镜还将配备一个教头，以便科学家可以使用该显微镜向本科生讲授最先进的组织学和免疫细胞化学技术。

Proposal Number: IBN-9418641 Principal Investigator: Cheryl Sisk This award is to provide a high-resolution epifluorescence microscope for research neuroendocrinologists at Michigan State University. High-resultion fluorescence microscopy will be used to localize biological molecules to specific subcompartments of individual cells within particular areas of the nervous system. These molecules are tagged with fluorescent markers that show particular colors under ultraviolet light. That way different molecules can be tagged with different colors to determine, for example, if a particular pair of molecules occur together or separately inside specific cells. These scientists will use the microscope for concurrent detection of activity-dependent markers and steroid receptor proteins, for comparisons of enzyme expression patterns in mast cells and glia, and for colocalization of neuropeptides and neurotransmitters in different regions of the brain. Furthermore, this technique can be used to determine how the distribution of these molecules changes when the animal is involved in different behaviors or, for example, is subjected to different photoperiods such as those that occur with the seasons. This microscope will also be equipped with a teaching head so that the scientists can use the microscope for teaching undergraduates these state-of-the-art histological and immunocytochemical techniques.