MRI: Changes in Uterine Gene Expression During Embryo Implantation in the Mouse

Yvette Huet-Hudson IBN编号9423382 这项研究的目的是检测暴露于雌激素后怀孕小鼠子宫中基因表达的变化。 雌激素是胚胎着床所必需的。 成功建立妊娠，导致健康啮齿动物后代的出生，取决于子宫内的正常着床。 着床是一个复杂的过程，受孕激素和雌激素的调节。 在孕甾酮预处理的子宫中，雌激素触发着床。 目前的一种理论是，孕激素/雌激素介导的胚胎着床调节是通过基因表达间接调节的。 研究还表明，胚胎需要在雌激素暴露后一小时内暴露于母体子宫才能着床。 为了更好地了解这一过程是如何启动的，研究人员将比较雌激素在雌激素预处理子宫中表达前和一小时后的基因。 这将通过产生子宫cDNA消减文库来实现。 这将允许研究者确定在成功植入所需的关键一小时时间段内，子宫中的雌激素打开或关闭了哪些基因。 在获得有关哪些基因的差异表达的信息，这是希望将奠定了基础，为未来的研究子宫生理在怀孕早期。 这位新的研究者是一位少数民族科学家，他在生殖生物学方面有很强的背景，包括最近在《国家科学院院刊》上发表的一篇论文。 她将利用这一倡议资金开始她的独立调查，为提交一份完整的提案做准备。

Yvette Huet-Hudson IBN#9423382 The objective of the proposed research is to examine changes in gene expression in the pregant mouse uterus following exposure to the hormone estrogen. Estrogen is required for embryo implantation. Successful establishment of pregancy, resulting in the birth of healthy rodent offspring, depends upon normal implantation in the uterus. Implantation is a complex process regulated by the hormones progesterone and estrogen. The initiation of implantation is triggered in the progesterone-primed uterus by estrogen. One current theory is that progesterone/estrogen mediated regulation of embryo implantation is modulated indirectly through gene expression. It has also been shown that embryos require exposure to the maternal uterus within one hour of estrogen exposure for implantation to occur. In order to better understand how this process is initiated, this investigator will compare the genes that are expressed prior to, and one hour after, estrogen in a progesterone-primed uterus. This will be accomplished by producing uterine cDNA subtraction libraries. This will allow the investigaor to determine which genes are turned on or turned off by estrogen in the uterus during the critical one hour time period required for successful implantation. In gaining information concerning which genes are differentially expressed, it is hoped that a foundation will be laid for future studies of uterine physiology during early pregnancy. This new investigator, a minority scientist, has a strong background in reproductive biology, including a recent publication in the journal Proceedings of the National Academy. She will use this initiative funding to begin her independent investigation, in preparation for the submission of a full proposal.