Equipment to Integrate Plant Tissue Culture into Plant Physiology and Plant Molecular Biology

路易斯安那州立大学什里夫波特分校植物生化和生理学课程的分子生物学部分将得到加强，为参加不同级别实验室课程的学生提供植物生理学和植物分子生物学实验室工作所需的细胞和组织培养设施。所要求的设备将用于一系列实验，旨在使学生能够制定和测试与理解植物生理学和分子生物学中的重要概念有关的假设。在100级和300级课程中，学生将研究启动愈伤组织生长和从愈伤组织再生植株所必需的植物激素比例的现象。参加400级别课程的学生将获得使用根癌农杆菌进行基因转化的经验，并使用植物组织进行基因调控实验，这些植物组织已在构成和热激启动子元件的控制下与GUS报告基因系统转化。除了在教学实验室课程中发挥关键作用外，所需的设备还将使从事研究项目的本科生能够进行个人调查。

The the molecular biology component of the plant biochemical and physiological curriculum at LSU-Shreveport will be enhanced by providing students enrolled in laboratory class at several levels with the cell and tissue culture facilities necessary for plant physiology and plant molecular biology laboratory work. The requested equipment will be used in a series of experiments designed to enable students to formulate and test hypotheses related to the understanding of important concepts in plant physiology and molecular biology. In the 100 and 300 level courses, students will investigate phenomena regarding the phytohormone ratios necessary to initiate the growth of callus tissue and to regenerate plants from callus tissue. Students enrolled in the 400 level class will gain experience in genetic transformations using Agrobacterium tumifaciens and conduct experiments in gene regulation using plant tissue that has been transformed with the GUS reporter gene system under the control of constitutive and heat shock promoter elements. In addition to serving a pivotal role in the instructional laboratory classes, the requested equipment also will enable undergraduates engaged in research projects to conduct individual investigations.