A Novel Technique to Identify Transgenic Drosophila

9505535坎比塞利斯博士请求为探索性研究小额赠款提供资金。该项目旨在开发一种实验方法，通过这种方法，特定的基因可以从一个果蝇物种的基因组转移到另一个物种的基因组中，而不需要像传统上那样在接受物种中使用这种遗传操作所需的遗传标记。尽管黑腹果蝇这一物种有大量的遗传突变体，但进化和发育生物学家感兴趣的大多数其他果蝇物种缺乏可用的突变体，限制了它们的遗传分析。为了纠正这种情况，便于分析夏威夷地方性果蝇和其他种群的基因表达和基因调控的进化，将开发以新霉素抗性或绿色荧光蛋白为标记的新型载体，以建立适用于任何果蝇物种的转化系统。这一方法论的成功发展将具有广泛的应用，例如促进对行为、进化等基本现象的遗传分析，以及模式形成的作用是发展。更广泛的应用可能确保转基因昆虫形成的一般方法随后可以扩展到具有经济重要性的昆虫，从而使解决农业问题的新方法成为可能。

9505535 Kambysellis Dr. Kambyselis requests funds for a small Grant for Exploratory Research (SGER). The project aims to develop an experimental methodology whereby specific genes can be transferred from the genome of one Drosophila species into another, without the use of genetic markers in the recipient species as traditionally required for such genetic manipulations. Whereas there is a vast array of genetic mutants available for the species Drosophila melanogaster, most other Drosophila species of interest to evolutionary and developmental biologist lack available mutants, limiting their genetic analysis. To rectify this situation and facilitate analysis of gene expression and the evolution of gene regulation in the endemic Hawaiian Drosophila and other groups, novel vectors using as markers neomycin resistance or the green fluorescent protein will be developed to establish a transformation system applicable to any Drosophila species. The successful development of this methodology will have broad application, facilitating genetic analyses of basic phenomena such as behavior, evolution, and the role of pattern formation is development, as examples. Even wider application may ensure if the general approach of transgenic insect formation can subsequently be extended to insects of economic importance, enabling novel approaches to agricultural problems.