Mechanisms of Electrode Reactions

这个项目得到了分析和表面化学计划的支持，旨在发展电化学方法来研究化学和生化问题。在这项为期三年的持续资助期间，德克萨斯大学奥斯汀分校的Allen J.Bard教授和他的学生将深入了解蛋白质结构、结合常数、化学计量学，并通过利用电活性物质与生物分子的相互作用来开发生物传感器。生物大分子与这些试剂相互作用引起的电流和电化学发光响应的变化将被用来探测涉及酶、抗体和DNA的反应。此外，扫描电化学显微镜(SECM)将被用来检测快速的多相和耦合的均相反应，并研究液-液界面上的电子和离子传输，从而能够表征聚合物薄膜和自组装单分子膜。几代人以来，电化学方法一直被用来测量许多金属、有机、生物和半导体物种的组成、浓度和反应活性。这些技术通常用于工业和临床行业，以进行简单的测定，如游泳池水的酸碱度、饮用水中的污染物，以及识别体内表明怀孕的复杂、微量的激素水平。德克萨斯大学奥斯汀分校的Allen Bard教授和他的学生一直是电分析化学领域的领导者，他们将在生物技术、环境分析和先进制造等领域引领新的分析和表面分析方法。

This project, supported in the Analytical and Surface Chemistry Program, is directed towards the development of electrochemical methods to study chemical and biochemical problems. During the tenure of this three-year continuing grant, Professor Allen J. Bard and his students at the University of Texas at Austin will gain insights into protein structure, binding constants, stoichiometry, and develop biosensors by exploiting the interaction of electroactive agents with biomolecules. Changes in current and electrochemiluminescence responses caused by interactions of biomacromolecules with these agents will be used to probe reactions involving enzymes, antibodies, and DNA. In addition, scanning electrochemical microscopy (SECM) will be used to detect fast heterogeneous and coupled homogeneous reactions and to investigate electron and ion transport across liquid-liquid interfaces, thus allowing characterization of polymer films and self-assembled monolayers. Electrochemical approaches have been used for generations to measure the composition, concentration, and reactivity of numerous metal, organic, biological and semiconductor species. These techniques are used routinely in industrial and clinical industries to make simple determinations such as the pH of water in swimming pools, contaminants in drinking water, and the identification of complex, trace level hormones in the body which signal pregnancy. Professor Allen Bard and his students at the University of Texas at Austin have been leaders in the field of electroanalytical chemistry and will be spearheading new analytical and surface analysis methods useful in such fields as biotechnology, environmental analysis, and advance manufacturing.