RPG: Molecular & Cellular Interphyletic Homology of Segmentation

[509099]接下来，研究人员将确定控制像果蝇这样的动物的发育的同源基因是否能控制像水蛭这样的动物的发育，这种动物同时产生所有的片段。她将研究可能参与组织水蛭胚胎（Helobdella triserialis）身体模式的基因的表达模式。待检测的基因是在果蝇（Drosophila melanogaster）的胚胎中表达的条带基因（stripco）中所包含的基因、cubitus interruptus-Dominant基因、无翅基因和hedgehog基因的同源基因，并且在该昆虫中已被证明是胚胎段正常发育所必需的。在水蛭胚胎中表达这些基因的细胞将通过它们的细胞谱系进行鉴定，并将通过激光消融来检查这些细胞在胚胎发育中发挥的作用。

9509099 Wedeen the investigator will determine whether homologs of genes that control development in an animal like the fruit fly, that generates all of its segments simultaneously, can control development in an animal like the leech, that adds segments progressively. She will examine the expression patterns of genes that are likely to be involved in organizing body pattern in the leech embryo, Helobdella triserialis. The genes to be examined are homologs of the genes engrailed, cubitus interruptus-Dominant, wingless and hedgehog that are expressed in stripco in the embryo of the fruit fly, Drosophila melanogaster, and have been shown in that insect to be required for normal development of embryonic segments. The cells in the leech embryo expressing these genes will be identified by their cell lineage and will be ablated by laser to examine what role these cells play in embryonic development.