Support for the Division of Environmental Biology PopulationBiology (DEB/PB) Program (REF) CPO-9216105 Task Order #85

9505535 Kambyselis博士为探索性研究（SGER）申请小额赠款。该项目旨在开发一种实验方法，通过这种方法可以将特定基因从一个果蝇物种的基因组转移到另一个果蝇物种中，而不需要像传统的遗传操作那样在受体物种中使用遗传标记。尽管黑腹果蝇有大量可用的基因突变体，但进化和发育生物学家感兴趣的大多数其他果蝇物种缺乏可用的突变体，限制了它们的遗传分析。为了改善这一状况，便于对夏威夷特有果蝇和其他类群的基因表达和基因调控进化进行分析，将开发以新霉素抗性或绿色荧光蛋白为标记的新型载体，建立适用于任何果蝇物种的转化体系。这种方法的成功发展将有广泛的应用，如促进行为、进化和模式形成在发展中的作用等基本现象的遗传分析。更广泛的应用可以确保转基因昆虫形成的一般方法随后可以扩展到具有经济重要性的昆虫，从而为解决农业问题提供新的方法。

9505535 Kambysellis Dr. Kambyselis requests funds for a small Grant for Exploratory Research (SGER). The project aims to develop an experimental methodology whereby specific genes can be transferred from the genome of one Drosophila species into another, without the use of genetic markers in the recipient species as traditionally required for such genetic manipulations. Whereas there is a vast array of genetic mutants available for the species Drosophila melanogaster, most other Drosophila species of interest to evolutionary and developmental biologist lack available mutants, limiting their genetic analysis. To rectify this situation and facilitate analysis of gene expression and the evolution of gene regulation in the endemic Hawaiian Drosophila and other groups, novel vectors using as markers neomycin resistance or the green fluorescent protein will be developed to establish a transformation system applicable to any Drosophila species. The successful development of this methodology will have broad application, facilitating genetic analyses of basic phenomena such as behavior, evolution, and the role of pattern formation is development, as examples. Even wider application may ensure if the general approach of transgenic insect formation can subsequently be extended to insects of economic importance, enabling novel approaches to agricultural problems.