Acquisition of Equipment for Research in Molecular Microbial Biology

该提案要求提供资金购买设备，以加强微生物系在分子微生物生物学领域的现有研究能力。申请的资金不仅将提供最先进的设备来加强该系的研究计划，而且还将加强该系在分子微生物生物学方面培训研究生和本科生的能力。P.I.(Mary E.Woodworth)和Co-P.I.(Luis A.Actis、J.K.Bhattacharjee、Joseph M.Carlin和Gary R.Janssen)在分子微生物生物学方面拥有完善的研究计划。此外，微生物学系的其他五名教员也有积极的研究计划，这些计划将受益于这种设备的使用。在这项提议的范围内进行的研究的重点是广泛的，因为它将包括以下方面的研究项目：病毒DNA复制的调控机制、细菌中铁获取系统的遗传和分子分析、酵母中赖氨酸生物合成的分子遗传学、吲哚胺2，3-双加氧酶(IDO)的调控分子机制以及细菌中无引线的mRNAs的翻译。学院教学和研究实验室对本科生和研究生的培养将变得更加复杂，适合当今对一流理科毕业生和具有科学素养的公民的特殊需求。为本科生和研究生开设的微生物和分子遗传学实验室课程将被描述为所需设备如何实现这些目标的一个例子。

This proposal requests funds to purchase equipment that will build upon and enhance existing research capabilities in the area of molecular microbial biology in the Department of Microbiology. The funds requested will not only provide state-of-the-art equipment to enhance the department's research programs, but will also strengthen the department's capabilities for training graduate and undergraduate students in the most modern techniques in molecular microbial biology. The P.I. (Mary E. Woodworth), and the Co-P.I.s (Luis A. Actis, J.K. Bhattacharjee, Joseph M.Carlin, and Gary R. Janssen) have well-developed research programs in molecular microbial biology. In addition, the five other faculty members in the Department of Microbiology have active research programs that will benefit from the use of this equipment. The focus of the research to be conducted within the scope of this proposal is broad in that it will encompass research projects on regulatory mechanisms of viral DNA replication, genetic and molecular analysis of iron acquisition systems in bacteria, the molecular genetics of Iysine biosynthesis in yeast, the molecular mechanisms of regulation of indoleamine 2,3-dioxygenase (IDO) and the translation of leaderless mRNAs in bacteria. The training of undergraduate and graduate students in the department's teaching and research laboratories will become more sophisticated and appropriate to today's special need for first-rate graduates in science and for scientifically literate citizens. The laboratory course in Microbial and Molecular Genetics for undergraduate and graduate students will be described as an example of how the requested equipment will accomplish these goals.