SGER: Montitoring Biological Processes in Single Living Cells

提案编号：IBN-9528526主要研究者：Day，Richard N. 细胞通过细胞内信号通路的变化对环境和发育刺激做出反应，从而导致基因表达模式的改变。 在活细胞中监测这些过程是生物学研究的主要挑战。 Day博士和他的同事们建议使用一种合成和分泌促性腺激素释放激素（GnRH）的神经元细胞系作为模型系统，以开发一种联合方法，同时监测单个活细胞中的基因表达，细胞内钙和电活动。 这些研究适用于探索性研究的小额赠款，因为建立从单个细胞确定所有这些细胞参数的技术具有高风险，并且这些研究对生物学研究具有重大影响。 如果这些实验成功，将对细胞将信号从细胞表面传递到细胞核的机制有新的理解。 这项研究的另一个影响是，如果成功的话，用于这些研究的方法将适用于几乎任何真核细胞系统的研究，提供独特的膜和核事件的时间联系。 将这项技术转移到生物体水平将使研究人员能够在自然环境中检查细胞，而不会损害它们的完整性，但可以明确地识别它们。

Proposal Number: IBN-9528526 Principal Investigator: Day, Richard N. Cells respond to environmental and developmental stimuli through changes in intracellular signaling pathways that lead to an altered pattern of gene expression. Monitoring of these processes as they occur in living cells is a major challenge for biological research. Dr. Day and his colleagues propose to use a neuronal cell line which synthesize and secrete gonadotropin-releasing hormone (GnRH) as a model system to develop a combined approach to simultaneously monitor gene expression, intracellular calcium and electrical activity in single living cells with temporal resolution. These studies are appropriate for a Small Grant for Exploratory Research because of the high risk associated with establishing the technology to ascertain all of these cellular parameters from a single cell and the significant impact of these studies to biological research. If these experiments are successful, new understanding will be forthcoming about the mechanisms used by cells to transmit signals from the cell surface to the nucleus. An additional impact of this research will be, if successful, the method used for these studies will be applicable to the study of virtually any eukaryotic cell system providing unique temporal linkage of membrane and nuclear events. The tpansfer of this technology to the organismal level will permit this investigator to examine cells in their natural surrounding without compromising their integrity, but permitting their unambiguous identification.