Redox regulation of the Nox2 and B7-H1 mRNA stability (A03)

我们以前的研究发现，mRNA的稳定性是氧化还原调节的一个目标。我们项目的第一个目标是鉴定调节SB7-H1 mRNA稳定性的RNA结合蛋白(RBP)(RNA-IP和MS，coop)。Z01项目)。然后对RBP的监管和功能进行了分析。第二个目标是表征RBP SYNCRIP的潜在氧化还原调节，在我们之前的研究中被确定为NOX2-mRNA结合蛋白。然后，我们使用建立的脓毒症小鼠模型来研究所分析的RBPs的表达变化如何调节其靶基因的表达，以及这是否对疾病的进程产生影响。

Our previous studies identified the mRNA stability as a target of redox regulation. First goal of our project is the identification of the RNA binding protein (RBP) that regulates the sB7-H1 mRNA stability (RNA-IP and MS, COOP. Z01 project). Followed by the regulatory and functional analysis of the RBP. Second goal is the characterization of the potential redox regulation of the RBP SYNCRIP, identified in our previous studies as a NOX2-mRNA binding protein. Afterwards we use the established sepsis mouse model to investigate how the alteration of the expression of the analyzed RBPs, can modulate the expression of their target genes and whether this has an influence on the course of the desease.