International Research Fellow Awards Program: Identification of AFLP Markers Linked to Disease Resistance Genes in the Commercial Strawberry

9600487海姆斯国际研究员奖励计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。该计划的奖励为联合研究提供了机会，并利用独特或互补的设施、专业知识和国外的实验条件。该奖项将支持Kenneth M. Haymes博士在荷兰瓦赫宁根植物育种与生殖研究中心（CPRO-DLO）与ir a . M. P. den Nijs博士进行为期12个月的博士后研究访问。Haymes博士和den Nijs博士将利用分子技术获得草莓基因组中与Pfr1、Pfr2和Pfr6基因相关区域的标记，这些基因赋予了草莓对致病性真菌疫霉（Phytophthora fragariae Hickman）（红根腐病）的抗性。他们还将研究现有草莓库存中是否存在这些标记，以制定合理的育种计划，以提高对疫霉感染的抗性。一种强大的定位技术，扩增片段长度多态性（AFLP）将在大量分离分析后使用，试图分离与Pfr1标记密切相关的标记。然后，将应用散装分离分析和AFLP来获得靠近其他抗性基因的标记。商品草莓是八倍体，因此分子标记是完成抗病和其他性状合理连锁研究的最大希望。一旦他们获得与增加抗真菌感染的基因紧密相连的标记，将对植物砧木进行检查，以确定适当的育种砧木，以生产含有多种抗性基因的草莓植株。＊＊＊

9600487 Haymes The International Research Fellow Awards Program enables U.S. scientists and engineers to conduct three to twentyfour months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit of Dr. Kenneth M. Haymes to work with Dr. ir A. M. P. den Nijs at the Centre for Plant Breeding and Reproductive Research (CPRO-DLO) in Wageningen, Netherlands. Dr. Haymes and Dr. den Nijs will use molecular techniques to obtain markers for regions of the strawberry genome that are linked to genes Pfr1, Pfr2 and Pfr6, which confer resistance to the pathogenic fungus Phytophthora fragariae Hickman (red stele root rot). They will also look at existing strawberry stocks for the presence of these markers to develop a rational breeding program for improved resistance to Phytophthora infection. A powerful mapping technique, amplified fragment length polymorphism (AFLP) will be used following bulked segregant analysis to attempt to isolate markers closely linked to the Pfr1 marker. Bulked segregant analysis and AFLP will then be applied to obtain markers near to the other resistance genes. The commercial strawberry is octaploid and thus molecular markers are the best hope for accomplishing rational linkage studies of disease resistance and other traits. Once they obtain markers that are tightly linked to genes which increase resistance to fungal infection, plant stocks will be examined for the purpose of identifying appropriate breeding stocks to produce strawberry plants containing multiple resistance genes. ***