Integrated Computer Crystallization Laboratories

加州大学旧金山分校生物化学系拥有一个充满活力的结晶学实验室。过去几年的成功鼓励该大学扩大结构生物学的教职。1995年，生物科学项目研究员Brigitte Raumann作为独立研究员加入。1996年，温德尔·林和乔纳森·韦斯曼加盟，担任细胞和分子药理学助理教授。这种扩张，以及在生物化学结构实验室工作的研究生和博士后研究员数量的自然增长，实际上已经使加州大学旧金山分校的X射线衍射和计算机设施不堪重负。在这份提案中，我们计划为生物化学系增加三维实验结构生物学的设施，并在细胞和分子药理学系内为一个新的实验室配备X射线衍射设备和计算机。拟议的新装备和更新实验室，一个是细胞和分子药理学，一个是生物化学，将在其使用和结构上，加州大学旧金山分校的所有X射线结晶学家都可以平等地进入。计划在三个层次上建立一个广泛的计算机系统，以便也能够以透明和同等的方式进行计算。在下面的叙述中，我们列出了六名首席调查员的项目，并证明了对新公共设备的需求。细胞和分子药理学系容纳新机器的实验室将进行翻新：将增加一堵墙，将核心计算机与X射线设备隔开，更换管道，安装电气设备和安全外壳。这些项目大多由美国国立卫生研究院提供给私人投资机构的拨款支持，以叙述性的形式简要描述，尽可能引用已发表的作品，并在后面列出定义项目和人员的表格。该表按标题列出了各个项目、从事该项目的主要科学家以及该项目的状况。《状态》简要概述了当前的数据收集需求。

UCSF has had a vibrant crystallography lab in the Department of Biochemistry. The successes over the last years encouraged the university to expand the Faculty Appointments in Structural Biology. In 1995, Brigitte Raumann, a Program in Biological Sciences Fellow, joined as an independent researcher. In 1996, Wendell Lim and Jonathan Weissman joined as Assistant Professors in Cellular and Molecular Pharmacology. This expansion, and the natural growth in numbers of graduate students and postdoctoral fellows working the structural labs in Biochemistry, has virtually overwhelmed the X-ray diffraction and computer facilities at UCSF. In this proposal we present plans to augment the facilities for three dimensional experimental Structural Biology for the Department of Biochemistry and to equip a new lab with X-ray diffraction equipment and computers within the Department of Cellular and Molecular Pharmacology. The proposed newly equipped and updated labs, one in Cellular and Molecular Pharmacology and one in Biochemistry, are to be in their use and structure, equally accessible to all X-ray crystallographers at UCSF. An extensive computer system built in three tiers is planned to permit computing also in transparent and equivalent ways. In the following narrative, we list the projects of the six principal investigators and demonstrate the need for the new communal equipment. The laboratory to house the new machines in the Department of Cellular and Molecular Pharmacology will be renovated: a wall is to be added for separating the core computers from the X-ray set, plumbing changed, electrical installation and a safety enclosure will be huilt. The projects, most supported by NIH grants to the P.I.s, are briefly described in narrative form, with references to published work where possible, and followed by a table defining projects and personnel. This lists the individual projects by title, the principal scientists working on the project and the status of the project. T he status provides a brief summary of the current needs for data collection.