Renovation and Consolidation of Salk Institute Plant Growth Facilities

该奖项将部分用于支持索尔克生物研究所植物生物学实验室（PBL）植物生长设施的翻新和巩固。主要研究方向包括：植物发育与生理学、植物发育的光调控、植物抗病性、植物生长控制的分子和遗传机制。 现有的生长设施被安置在金属结构中，不足以提供支持科学计划所需的功能和环境条件。 翻修将包括：结构改善，升级暖通空调，管道和电气系统，安装橱柜，新的灯具和电线架的工厂。 一旦完成，植物准备，生长和分析的不同功能将被物理分离。 集中式植物生长设施的优化布局将为生长室提供可用的架子空间，主要用于种植拟南芥植物。 在升级后的设施中进行的具体项目包括：特殊光照条件下的突变体筛选，使用转基因植物和已知调控基因的弱突变等位基因的第二代突变体筛选，以及通过功能获得分析识别新调控基因的大规模激活标记筛选。 此外，该项目还将用于遗传分析、绘图和生物化学研究所需的一般植物生长和维持，用于生产转基因植物和植物的无病生长。

This award will be used to partially support the renovation and consolidation of the Plant Growth Facilities of the Plant Biology Laboratory (PBL) at the Salk Institute for Biological Studies. Research interests, represented by four groups, include: the development and physiology, light regulation of plant development, plant disease resistance, and molecular and genetic mechanisms of growth control. Existing growth facilities are housed in a metal structure inadequate to provide the required functional and environmental conditions to support scientific programs. Renovations will consist of: structural improvements, upgrading the HVAC, plumbing, and electrical systems, installing casework, new light fixtures and wire racks for plants. Once completed, the different functions of plant preparation, growth, and analysis will be physically separated. The optimized layout of the centralized Plant Growth Facility will provide usable shelf space in the growth rooms, mainly for growing Arabidopsis plants. Specific projects to be conducted in the upgraded facility include: mutant screens under special light conditions, second-generation mutant screens using transgenic plants and weak mutant alleles of known regulatory genes, and large-scale activation-tagging screens to identify new regulatory genes through gain-of-function analyses. In addition, the project will be used for general plant growth and maintenance as required for genetic analyses, mapping purposes and biochemical studies, for the generation of transgenic plants, and for disease-free growth of plants.