A Rotating Anode X-ray Generator for Biological Macromolecular Crystallography

需要资金购买一台旋转阳极X射线发生器，以便测量生物大分子晶体的衍射数据。该仪器将为我们现有的成像板探测器系统提供急需的X射线源，并将直接支持3位主要研究人员的研究以及蛋白质晶体学的教学。将利用这种X射线源的项目包括DNA甲基化;博来霉素水解酶及其复合物;转录复合物;和RNA剪接因子，所有这些晶体目前都可用。 在这些项目的过程中阐明的晶体结构将提供结构框架，沿着生物化学和遗传学。 研究，以解释功能和 这些不同范围的生物化学和生物医学上重要的大分子的分子特性。此外，该设备是必要的教学在高分子晶体学在每年秋季提供在冷泉港实验室，以及培养我们自己的研究生和博士后研究员。 大分子晶体学的高级课程对结构生物学领域有着广泛的影响，因此除了对这里描述的特定晶体学研究的重要性外，正常运行的X射线设备对大分子晶体学领域的价值通常是巨大的。

Funds are requested to purchase a rotating anode X-ray generator in order to measure diffraction data from biological macromolecular crystals. This instrument will provide a source of X-rays urgently needed for our existing imaging plate detector system and will directly support the research of 3 major investigators as well as instruction in protein crystallography. Projects that will utilize this X-ray source include DNA methylation; the bleomycin hydrolases and their complexes; transcription complexes; and RNA splicing factors, for all of which crystals are currently available. The crystal structures elucidated in the course of these projects will provide structural frameworks, along with the biochemical and genetic studies, for interpreting the functional and molecular properties of this diverse range of biochemically and biomedically significant macromolecules. In addition, the equipment is necessary for teaching an advanced course in macromolecular crystallography offered in the fall of every year at Cold Spring Harbor Laboratory as well as training our own graduate students and postdoctoral fellows. The advanced course in macromolecular crystallography has a wide impact on the field of structural biology and thus in addition to its importance for the specific crystallographic studies described here, the value of a properly operating X-ray facility to the field of macromolecular crystallography in general would be substantial.