Gene Mapping by Pulsed Field Gel Electrophoresis

随着脉冲场凝胶电泳（PFGE）的出现，现在可以很容易地操纵DNA的大片，从而促进MAR映射。马萨诸塞州总医院内的三个独立研究项目正在着手进行这种绘图。第一个是以卡尔·S·Shelley，将鉴定与CD43基因相关的MAR，第二个，以M. Amin Arnaout将绘制那些与编码整合素家族的基因相关的图谱，第三个整合素家族由Mason W.弗里曼将绘制清道夫受体基因侧翼的MAR。CD43和α链2整联蛋白基因编码介导白细胞粘附功能的分子。由于这些基因聚集在16号染色体的pll.2区域，因此Shelley博士和Arnaout博士的研究小组也将使用PFGE来生成该簇的物理图谱。预计在该簇内的MAR的映射将定义转录结构域，并为确定体内控制这些功能相关基因的协调和差异调节的分子机制提供基础。Shelley、Arnaout和Freeman博士目前无法使用PFGE系统，因此需要国家科学基金会提供资金购买。

With the advent of Pulsed Field Gel Electrophoresis (PFGE) large stretches of DNA can now be readily manipulated thereby facilitating MAR mapping. Three independent research projects within Massachusetts General Hospital are embarking on such mapping. The first, headed by Carl S. Shelley, will identify the MARs associated with the CD43 gene, the second, headed by M. Amin Arnaout will map those associated with the genes encoding the, 2 integrin family and the third, headed by Mason W. Freeman, will map the MARs which flank the scavenger receptor gene. The CD43 and alpha chain 2 integrin genes encode molecules which mediate the adhesive functions of leukocytes. Since these genes are clustered on the pll.2 region of chromosome 16, PFGE will also be used by Dr. Shelley's and Dr. Arnaout's groups to generate a physical map of this cluster. It is anticipated that mapping of the MARs within this cluster will define transcriptional domains and provide the basis for determining the molecular mechanisms which in vivo control the coordinate and differential regulation of these functionally related genes. Drs. Shelley, Arnaout and Freeman currently do not have access to a PFGE system and here funding is requested of the National Science Foundation for its purchase.