RUI: Lipid Signaling During Fertilization

摘要/ Abstract摘要：受精过程涉及一系列膜脂融合事件，但脂质在受精过程中作为细胞间信号的作用在很大程度上尚未被探索。PI表明，精子增加了爪蟾卵中由磷脂酶c产生的肌醇-1,4,5-三磷酸的数量。PI还证明磷脂酶D在受精过程中也起作用。这种酶的产物胆碱在受精时增加，而这种酶的抑制剂会抑制受精。这项拨款的实验将继续研究磷脂酶D在各种受精事件中的作用。这项研究将增加对受精过程中发生的脂质变化的认识。＊＊＊

Abstract 9631051 Stith Fertilization involves a series of membrane lipid fusion events, however the role of lipids as intercellular signals during the process of fertilization is largely unexplored. The PI has shown that sperm increases the amount of inositol -1,4,5-triphosphate in the Xenopus egg, produced by the action of phospholipase C. the Pi has evidence that phospholipase D also plays a role in fertilization. Choline, the product of this enzyme, increases at fertilization, and an inhibitor of the enzyme inhibits fertilization. The experiments in this grant will pursue studies on the action of phospholipase D in various fertilization events. This research will increase knowledge of the changes in lipids that occur during fertilization. ***