RPG: A Genetic Approach to the Analysis of Intracellular Diacylglycerol Transport

9707108 Trotter这是一个RPG项目，将为PI提供一个机会，利用酵母中的遗传方法开发关于甘油二酯转运的研究项目。 DAG是脂质代谢中的重要中间体，并参与细胞信号传导。 DAG在细胞内的各种膜之间移动，但介导这种移动的机制尚不清楚。 将使用荧光DAG衍生物开发突变体筛选，以发现运输过程中的突变体。 最终，相关的基因可以被识别和研究。 这是一个研究计划资助项目，将探索用于识别膜间脂质转运缺陷的酵母突变体的方法。 这些化合物对于膜功能和细胞信号传导是重要的。 这项工作可能会提供有关如何协调和调节合成的新信息。 ***

9707108 Trotter This is an RPG project which will give the PI an opportunity to develop a research project on diacylglycerol transport using a genetic approach in yeast. DAG is an essential intermediate in lipid metabolism and is involved in cell signaling. DAG moves between various membranes in the cell, but the mechanisms that mediate the movement are not known. A mutant screen will be developed using a fluorescent DAG derivative to find mutants in the transport process. Eventually, the genes involved can be identified and studied. %%% This is a Research Planning Grant project that will explore the development of methods for identifying yeast mutants that are defective in lipid transport between membranes. These compounds are important for membrane function and cell signaling. This work may provide new information about how synthesis is coordinated and regulated. ***