RPG: A Genetic Approach to the Analysis of Intracellular Diacylglycerol Transport

9707108 Troter这是一个RPG项目，它将使PI有机会开发一个在酵母中使用遗传方法进行二酰甘油运输的研究项目。DAG是脂质代谢的重要中间产物，参与细胞信号转导。DAG在细胞内的各种膜之间移动，但调节这种移动的机制尚不清楚。将使用荧光DAG衍生物开发突变筛选，以寻找运输过程中的突变。最终，涉及的基因可以被识别和研究。%这是一个研究计划资助项目，该项目将探索开发鉴定酵母突变株的方法，这些突变株在膜之间的脂质运输方面存在缺陷。这些化合物对膜功能和细胞信号转导很重要。这项工作可能会提供有关合成如何协调和调节的新信息。***

9707108 Trotter This is an RPG project which will give the PI an opportunity to develop a research project on diacylglycerol transport using a genetic approach in yeast. DAG is an essential intermediate in lipid metabolism and is involved in cell signaling. DAG moves between various membranes in the cell, but the mechanisms that mediate the movement are not known. A mutant screen will be developed using a fluorescent DAG derivative to find mutants in the transport process. Eventually, the genes involved can be identified and studied. %%% This is a Research Planning Grant project that will explore the development of methods for identifying yeast mutants that are defective in lipid transport between membranes. These compounds are important for membrane function and cell signaling. This work may provide new information about how synthesis is coordinated and regulated. ***