CAREER: Steroid Regulation of Development in Drosophila

小行星9722205 类固醇激素控制着高等生物的广泛发育过程，与受体蛋白共同调节靶基因的阶段特异性和组织特异性转录。关于这些对基因表达的影响如何导致与果蝇（Drosophila melanogaster）中类固醇功能相关的显著发育变化，人们知之甚少。这项研究的目的是回答一个单一的激素信号如何在发育过程中的不同时间引起不同的反应。拟进行的研究将探讨20-羟基蜕皮激素（蜕皮激素）在果蝇变态的发病过程中触发的监管层次。 先前分离以及新分离的突变体BFTZ-F1的功能丧失突变将进行分析，重点是唾液腺组织溶解，蛹角质层形成和头部外翻。还将在分子水平上表征突变。 在分子水平上理解生物过程导致了我们对生物过程背后机制的理解的快速，重大进展，这种理解对日常世界产生了重大影响。因此，它是至关重要的学生获得分子生物学的坚实的理解，无论他们希望成为科学家，或知情的公民谁是装备精良，以处理有关使用科学知识的个人和社会决策。在这项研究中，本科生将参与分析类固醇激素在触发果蝇（Drosophila melanogaster）发育过程中的作用机制。 分子生物学课程整合方案是生物科学原理教学的有效途径。 ***

9722205 Woodard Technical section steroid hormones control a wide range of developmental processes in higher organisms, acting in conjunction with receptor proteins to regulate the stage- and tissue-specific transcription of target genes. Little is understood about how these effects on gene expression result in the dramatic developmental changes associated with steroid function in the fly, Drosophila melanogaster. The goal of the proposed research is to answer the question of how a single hormonal signal can elicit different responses at different times during development. The proposed study will examine the regulatory hierarchies triggered by 20-hydroxyecdysone (ecdysone)during the onset of Drosophila metamorphosis. Previously isolated as well as newly isolated loss-of-function mutations in a mutant, BFTZ-F1 will be analyzed with a focus on salivary gland histolysis, pupal cuticle formation, and head eversion. The mutations will also be characterized at the molecular level. Non-technical Understanding biological processes at the molecular level has led to rapid, major advances in our understanding of mechanisms underlying biological processes and that understanding has had a major impact in the everyday world. Thus it is critical for students to gain a solid understanding of molecular biology, whether they wish to become scientists, or informed citizens who are well-equipped to deal with personal and societal decisions regarding use of scientific knowledge. In this study, undergraduate students will be involved in the analysis of the mechanism(s) involved in the function of the steroid hormone in triggering developmental processes in the fruit fly, Drosophila melanogaster. The plan to integrate molecular biology into the curriculum is an effective way of teaching scientific principles of biology. ***