Electrochemical Biosensors Based on Quinoprotein Enzymes

该研究项目由分析和表面支持， 化学计划，专注于quinoprotein的开发 用于分析组胺，多巴胺， 和酒精。 杰克逊州立大学的立川教授 和密西西比大学医学中心的戴维森， 他们的学生将学习准备和表征 使用甲胺脱氢酶的生物传感器， 芳香胺脱氢酶和甲醇脱氢酶。 电化学方法，电化学石英晶体 将使用微量天平测量和拉曼光谱 为了表征这些酶的结构和功能， 生物传感器这个项目是独一无二的，在使用氧气和NAD 生物传感器中的独立氧化还原酶， 预期会有医疗应用。 本项目将研究开发基于酶的生物传感器的实用性，该传感器使用氧和NA独立的醌蛋白酶。 杰克逊州立大学的Tachikawa教授和密西西比大学医学中心的Davidson教授将与他们的学生一起研究这些生物传感器的构建和表征。 一个广泛的，跨学科的方法，这个项目将涉及使用电化学技术，石英晶体微量天平测量，和拉曼光谱，以确定这些新的生物传感器的结构和性能。 长期目标集中于开发定量和定性分析在医学中的新应用。

This research project, supported by the Analytical and Surface Chemistry Program, focusses on the development of quinoprotein enzyme based biosensors for the analysis of histamine, dopamine, and alcohols. Professors Tachikawa of Jackson State University and Davidson of The University of Mississippi Medical Center and their students will study the preparation and characterization of biosensors using the enzymes methylamine dehydrogenase, aromatic amine dehydrogenase, and methanol dehydrogenase. Electrochemical methods, electrochemical quartz crystal microbalance measurements, and Raman spectroscopy will be used to characterize the structure and function of these enzyme based biosensors. This project is unique in using oxygen and NAD independent redox enzymes in biosensors and in the long-term medical applications are anticipated. This project will examine the utility of developing enzyme based biosensors that use oxygen and NA independent quinoprotein enzymes. Professors Tachikawa of Jackson State University and Davidson of The University of Mississippi Medical Center will study the construction and characterization of these biosensors with their students. A broad, interdisciplinary approach to this project will involve use of electrochemical techniques, quartz crystal microbalance measurements, and Raman spectroscopy to determine the structure and properties of these new biosensors. Long-term goals focus on developing new applications for quantitative and qualitative analysis in medicine.