Improvement of a Biochemistry Laboratory Course

中康涅狄格州立大学的科学课程旨在为学生提供高质量的指导和最先进的实验室经验，为他们在工业和教育领域的职业生涯或进一步的研究生教育做准备。化学学士学位是由美国化学学会专业培训委员会(ACS CPT)认证的。为了满足学生的兴趣，并使学生更好地为工业做好准备，我们正在开发符合ACS CPT指南的生物化学专业。为了实现这一目标，我们必须(1)将目前的生物化学课程从3学分延长到6学分；(2)通过融入现代技术和更多创新实验，提高我们3小时生物化学实验室课程的范围和质量；以及(3)为对生物化学感兴趣的本科生提供更多的研究机会。本提案旨在通过购买用于修订的阶段性高压液相色谱(HPLC)、高速低温离心法和组织匀浆的仪器来改善生化实验室计划。这些仪器将在化学系的下列课程中使用：生物化学实验室和基础有机与生物化学；生物系的生态生理学。教授这些课程的教授还将在涉及本科生的个人研究项目中使用这些工具。琼斯博士是化学系的生物化学家，他正在研究多芳烃和卤代烃在原核生物和真核细胞中的代谢和毒性。她最近建立了一个小型组织培养实验室，用于在原代培养的大鼠肝细胞中进行代谢研究。Kapper博士是生物系的比较生理学家，致力于研究海洋和河口有机体盐度适应的生理生化机制。这些研究将通过获得高效液相技术而得到加强。具体地说，我们建议购买Waters反相高效液相系统，具有紫外/可见光和荧光检测能力。这将用于分析蛋白质水解物、氨基酸和其他生物分子，以及在植物和动物组织中产生的环境代谢物。Sorval RC-26 Plus离心机(最大速度26,000rpm)和铝制定角转子，将用于哺乳动物组织的亚细胞分离，包括用于代谢研究的钙沉淀微粒体亚细胞分离，以及特定细胞蛋白质的梯度纯化。组织匀浆器将用于产生组织匀浆，用于差速离心法。购买这些仪器将代表着化学系内生物化学培训的质量和范围的重大进步，并对这一领域拟议的计划发展做出重大贡献。高效液相系统将被广泛应用于化学系和生物科学系的其他以及本科生研究项目。

Science programs at Central Connecticut State University seek to provide students with high quality instruction and state of the art laboratory experiences in preparation for careers in industry and education or further postgraduate education. The B.S. degrees in Chemistry are certified by the American Chemical Society's Committee on Professional Training (ACS CPT). In response to student interest and to better prepare students for industry, we are developing a specialization in Biochemistry consistent with the ACS CPT guidelines. To achieve this goal we must (1) extend our current biochemistry lecture course from 3- to 6-credit hours; (2) improve the scope and quality of our 3-hour biochemistry laboratory program by the incorporation of modern technology and more innovative experiments; and (3) provide more research opportunities for undergraduates interested in biochemistry. The present proposal addresses our intent to improve the biochemistry laboratory program through the purchase of instrumentation for revised phased high pressure liquid chromatography (HPLC), high speed, low temperature centrifugation, and tissue homogenization. The instruments will be utilized in the following courses in the Chemistry Department: Biochemistry Laboratory and Basic Organic and Biological Chemistry; and in the Biology Department, in Ecological Physiology. The professors who instruct these courses will also utilize the instrumentation in their individual research programs which involve undergraduates. Dr. Jones, a biochemist in the Chemistry Department, is studying the metabolism and toxicity of polyaromatic hydrocarbons and halogenated hydrocarbons in prokaryote and eukaryote cells. She has recently established a small tissue culture laboratory for conducting metabolic studies in primary rat liver cell cultures. Dr. Kapper, a comparative physiologist in the Biology Department, is engaged in study of the physiological and biochemical mechanisms of salinity adaptation in marine and estuarine orga nisms. These studies would be enhanced by access to HPLC technology. Specifically, we propose to purchase a Waters reversed phase HPLC system with capability for uv/visible and fluorescence detection. This will be used for analyses of protein hydrolysates, amino acids and other biomolecules, and environmental metabolites generated in plant and animal tissues. The Sorval RC-26 Plus centrifuge (max. speed 26,000rpm) with and aluminum fixed-angle rotor, will be used for the subcellular fractionation of mammalian tissues including calcium-precipiated microsomes for metabolic studies, and for the gradient purification of specific cellular proteins. A tissue homogenizer will be used to generate tissue homogenates for differential centrifugation. The acquisition of these instruments would represent a major advancement in the quality and scope of training in biochemistry within the Chemistry Department, and contribute significantly to the proposed program development in this area. An HPLC system would be widely used in other as well as undergraduate research projects in the Departments of Chemistry and Biological Sciences.