SBIR Phase I: Solid State Instrumentation for Nucleic Acid Quantification

这是一项小企业创新研究的第一阶段提案。我们建议开发用于快速准确定量核酸浓度的仪器和技术。我们相信我们的方法可以被许多分子生物学实验室用于寡核苷酸、质粒、总RNA、MRNA或基因组DNA的常规测量。该仪器的主要组成部分是基于固态芯片上非常灵敏的磁场传感器的检测模块。核酸的量是通过测量与核酸结合的探针产生的磁场来确定的。在I期研究中，我们将改进我们的原型并使用寡核苷酸、质粒、总RNA、MRNA和/或基因组DNA测试系统。我们的目标是生产一种能够可靠地检测一微升体积中一纳克核酸的仪器。我们预计该仪器将用于基础研究，以检测少量的核酸。我们还希望生产一种便携式设备，用于检测供水和食品加工材料中的生物制剂。

This is a Small Business Innovation Research Phase I proposal We propose to develop instrumentation and techniques for the rapid and accurate quantification of nucleic acid concentrations. We believe that our method could be used by many molecular biology labs for routine measurements of oligonucleotides, plasmids, total RNA, MRNA or genomic DNA. The major component of this instrument is a detection module based on a very sensitive magnetic field sensor on a solid-state chip. The amount of nucleic acid is determined by measuring the magnetic field generated by probes bound to the nucleic acid. In Phase I studies we will improve our prototype and test the system with oligonucleotides, plasmids, total RNA, MRNA and/or genomic DNA. Our goal will be to produce an instrument which can reliably detect one nanogram of nucleic acid in a one microliter volume. We anticipate this instrument will be used in basic research to detect small amounts of nucleic acids. We also hope to produce a portable device for the detection of biological agents in water supplies and food processing materials.