Accessioning and Curation of the ATCC Bacteriology Collection

细菌学收藏是世界上最多样化的细菌组合之一。它目前拥有近18,000个菌株，代表大约600个公认的属。大约20%的菌株加入了NSF支持（“NSF支持培养”）。1997年，超过19,000瓶nsf支持的培养在美国和世界各地分发。这些培养物被用于各种应用：教学、质量控制测试、工业生产和研究。此外，细菌收集被认为是标准参考培养物的存储库，菌株在许多手册和程序中列出。认证和质量控制一直并将继续在收藏的最高标准执行。大多数菌株已经通过一套生理、形态和细胞化学特征被广泛识别，这些特征是该物种特有的。新的分子方法正在被评估纳入常规质量控制程序，以实现细菌菌株特征的多相分类方法。该提案是NSF BIR-9512045的延续，该提案支持大约13%的所需总支出，用于持续维护收藏并提高其收购质量。这些资金还将在未来几年内为该组织建立标准化的微生物鉴定资源提供部分支持。

The Bacteriology Collection is one of the most diversified assemblages of bacteria in the world. It currently holds close to 18,000 strains representing approximately 600 recognized genera. Approximately 20% of total strains were accessioned with NSF support (the "NSF-supported cultures"). In 1997, more than 19,000 vials of NSF-supported cultures were distributed in the U.S. and throughout the world. These cultures were used in a variety of applications: teaching, quality control testing, industrial production, and research. In addition, the Bacterial Collection is recognized as a repository for standard reference cultures, and the strains are listed in a number of manuals and procedures.Authentication and quality control have been and continue to be performed at the highest standard in the Collection. The majority of the strains have been identified extensively by a suite of physiological, morphological, and cytochemical characteristics that are specific to that species. New molecular methods are being evaluated for incorporation as routine quality control procedures to achieve a polyphasic taxonomic approach in the characterization of bacterial strains.This proposal is a continuation of NSF BIR-9512045, which supports approximately 13% of the total expenditures required-for the on-going maintenance of the Collection, and for enhancing the quality of its acquisitions. These monies will also provide partial support for the Collection to set up standardized resources for microbial authentication in the next few years.