CAREER: The Design of Synthetic Gene Transfer Agents

这项建议的目标是开发足够的脂质体和合成聚合物载体的结构和动态数据，以允许设计更有效的基因转移剂。理论模型包括：两种载体类型的相图，确定作为系统参数的函数的平衡结构；检查可能的非平衡结构及其形成条件；以及利用结构/功能关系关联各种载体的性能。本建议的最终目标是识别和量化控制通过合成载体进行基因转移的基本过程。

The goal of this proposal is to develop sufficient structural and dynamic data on liposome-based and synthetic polymer-based vectors to permit the design of more efficient gene transfer agents. A theoretical model will be derived from the data which includes:Phase diagrams of both carrier types identifying the equilibrium structures as a function of system parameters;Examining possible non-equilibrium structures and the conditions for their formation; and Relating the performance of the various carriers using structure/function relationships.The ultimate goal of this proposal is to identify and quantify the fundamental processes controlling gene transfer via synthetic carriers.