Subcellular interactions between Ca2+ and cAMP in determining neuronal activity

呼吸网络由神经元组成，其起搏和突触活动严重依赖于两个第二信使，Ca 2+和cAMP。它们的水平通过特定离子通道的性质的改变由神经元活动调节。Ca ~（2+）、cAMP和离子通道之间的相互作用可能发生在细胞膜、线粒体、内质网和细胞核附近的微米级亚细胞区域。我们的目的是研究这些相互作用，在呼吸神经元相结合的钙离子和cAMP成像与电生理。遗传编码的荧光探针将在含有呼吸网络的器官型切片的神经元中特异性表达。我们将研究亚细胞内Ca 2+和cAMP水平的波动与单离子通道和神经元活动模式的活动有关，以及这种信号在发育和成熟过程中如何变化。这些知识对于理解控制神经元活动和可塑性的机制非常重要，这些神经元活动和可塑性是呼吸等重要功能的控制机制。

The respiratory network consists of neurons whose pacemaker and synaptic activities are critically dependent on the two second messengers, Ca2+ and cAMP. Their levels are modulated by neuronal activity through modification of properties of specific ion channels. The interactions between Ca2+, cAMP and ion channels are likely to take place in micrometer-wide subcellular domains which can be located in the vicinity of plasma membrane, mitochondria, endoplasmic reticulum and nucleus. We aim to examine these interactions in respiratory neurons by combining Ca2+ and cAMP imaging with electrophysiology. Genetically encoded fluorescent probes will be specifically expressed in neurons of organotypic slices containing the respiratory network. We will examine subcellular fluctuations of Ca2+ and cAMP levels as related to the activity of single ion channels and neuronal activity pattems and how this signalling changes during development and maturation. This knowledge is important for understanding the mechanisms controlling neuronal activity and plasticity in such vital function as breathing.