Postdoctoral Research Fellowship in Microbial Biology for FY 2000

该行动资助了2000年美国国家科学基金会微生物生物学博士后研究奖学金。该研究和培训计划是在微生物遗传学领域，题为“古细菌同源重组机制”。本研究探讨了嗜热古细菌Solfolobus solfataricus的一般重组机制。真核重组蛋白的功能同源物正在异种生产，并用于体外重建古细菌重组。体内基因破坏和突变体拯救研究将补充体外数据，并证明S. solfataricus产生的蛋白质与酵母产生的蛋白质在功能上的同源性。同源重组是一种普遍的过程，其中遗传信息在不同的DNA链之间交换，引入新的连接和遗传序列。

This action funds an NSF Postdoctoral Research Fellowship in Microbial Biology for 2000.The research and training plan is in the area of Microbial Genetics and is entitled "Mechanism of Homologous Recombination in Archaea". This research examines general recombination mechanisms in the hyperthermophilic archaeal microbe Solfolobus solfataricus. Functional homologues to eukaryotic recombination proteins are being heterologously produced and are used to reconstitute archaeal recombination in-vitro. In-vivo gene disruption and mutant rescue studies will complement the in-vitro data and demonstrate functional homology of proteins produced by S. solfataricus to those produced in yeasts. Homologous recombination is a universal process in which genetic information is exchanged between separate strands of DNA, introducing new linkages and genetic sequences.