Postdoctoral Research Fellowships in Microbial Biology for FY 2000

这一行动资助了NSF 2000年微生物生物学博士后研究奖学金。该研究和培训计划是在生态和进化生理学领域，题为“极小藻类的多样性和生理学”。这项研究解决了不同海洋蓝藻分支的存在在生态位适应方面的差异所起的作用的问题。正在对海洋蓝藻聚球藻的两个自由生活菌株进行比较，一个代表沿海分支，另一个代表远洋分支，以评估生理差异和对氮饥饿的适应能力。目前正在构建消减文库，并对序列进行分析，以确定硝酸盐转运蛋白的蛋白质序列和基序。产生的序列被用来制作微阵列，芯片实验被设计来检查和比较这两个菌株对氮剥夺的幅度和动力学。这些数据将有助于回答这样一个问题，即不同的菌株在表达模式上是否存在差异，表明生态位适应或资源划分，这两者都是对竞争的频繁进化反应。

This action funds an NSF Postdoctoral Research Fellowship in Microbial Biology for 2000.The research and training plan is in the area of Ecological and Evolutionary Physiology and is entitled "Diversity and Physiology of Extemely Small Algae." This research addresses the question of what role differences in niche adaptation play in the existence of different clades of marine cyanobacteria. Two free-living strains of the marine cyanobacterium Synechococcus, one representing a coastal clade and the other an open ocean clade, are being compared to assess physiological differences and adaptive capacities to nitrogen starvation. Subtraction cDNA libraries are being produced and the sequences analyzed to identify protein sequence and motifs of nitrate transporting proteins. Generated sequence is used to produce a microarray, and chip experiments are designed to examine and compare the magnitude and kinetics of the two strains to nitrogen deprivation. This data will help answer the question of whether different strains differ in expressional patterns in ways that indicate niche adaptation or resource partitioning, both of which are frequent evolutionary responses to competition.